纳米硒桔梗多糖复合物对CCl_4致小鼠肝损伤的保护作用  

Protective effects of nano-selenium/platycodon polysaccharides complex on CCl_4-induced liver damage in mice

作　　者：侯巍[1] 侯丽然[2] 张云杰[1] 郑晓凤[1] 李贵森 彭翔 高金波[1] 

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007 [2]佳木斯大学基础医学院,黑龙江佳木斯154007 [3]多多药业有限公司,黑龙江佳木斯154007

出　　处：《食品工业科技》2018年第1期308-311,317,共5页Science and Technology of Food Industry

基　　金：黑龙江省卫生厅项目(2016-318);黑龙江省中医管理局(ZHY16-115);佳木斯大学研究生科技创新项目(YM2016_091)

摘　　要：以桔梗多糖和Na2Se O3为原料,采用维生素C还原法制备纳米硒桔梗多糖复合物。建立CCl_4小鼠化学损伤肝模型,通过检测小鼠血清生化指标和肝组织中丙二醛(MDA)的含量和谷胱甘肽过氧化物酶(GSH-Px)的活性,计算肝脏指数,观察肝组织病理学变化;评价纳米硒桔梗多糖复合物对CCl_4致小鼠肝损伤的保护作用。结果表明:各给药组与模型组相比,小鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)和碱性磷酸酶(ALP)水平有不同程度的降低;总蛋白(TP)和白蛋白(ALB)的含量有不同程度升高。同时,组织中GSH-Px活力显著(p<0.05)升高,MDA含量显著(p<0.05)降低。病理切片显示,纳米硒桔梗多糖复合物各给药组可不同程度地保护肝脏细胞避免CCl_4的损害。证实纳米硒桔梗多糖复合物对CCl_4致小鼠肝损伤有一定的保护作用且强于桔梗多糖,为开发新型的含硒保肝药物提供了一定的科学依据。Using platycodon Polysaccharide and Na2SeO3 as raw materials, the complex containing nano-selenium and platycodon polysaccharide was prepared by reduction method by virtue of vitamin C as reductant.The mice model with injury liver induced by CC14 was founded.By detection of biochemical indicator of blood serum, malondialdehyde（ MDA ）content and glutathione peroxidase（GSH-Px） activity in liver tissue, the liver index was calculated. According to the histopathological changes in liver,the protective effect of nano-selenium/platycodon polysaccharide complex on liver injury induced by CC14 in mice was evaluated.The results indicated that alanine aminotransferase（ALT）, aspartate aminotransferase（AST） and alkaline phosphatase （ALP）in the serum of mice were lower than those of the control group.Total protein （TP）and albumin （ ALB ）levels was observed to increase in varying degrees.At the same time,The activity of glutathione peroxidase（GSH-Px） in the tissues significantly （ p 〈 0.05 ） increased, while the content of malondialdehyde （ MDA ） decreased significantly （p 〈 0.05 ） . For medication group,it was shown by pathological sections that SePP can protect liver cells fi＇om damage by CCI4 chemicals.It was confirmed that the complex containing nano - selenium and platycodon presented stronger protective effect on liver injury induced by CC14 in mice than that of platycodon polysaccharide.It paves a way to the development of new selenium containing liver protecting drugs.

关 键 词：纳米硒 桔梗多糖 保肝 CCL4 

分 类 号：TS201.4[轻工技术与工程—食品科学]