基于表面增强拉曼技术快速检测5种食源性致病菌  

Quick Detection of Five Foodborne Pathogenic Bacteria Based on Surface Enhanced Raman Spectroscopy

作　　者：高玮村 李博[2] 王习文[2] 李乾学[2] 李楠[2] 李志萍[2] 夏志平[1,2] 

机构地区：[1]吉林农业大学动物科学技术学院,长春130118 [2]军事医学科学院军事兽医研究所,长春130122

出　　处：《吉林农业大学学报》2017年第6期733-737,共5页Journal of Jilin Agricultural University

基　　金：国家重点研发计划项目(2016YFD501001);武汉东湖新技术开发区第七批"3551光谷人才计划"

摘　　要：为快速检测布鲁氏菌S2株、鼠伤寒沙门氏菌、金黄色葡萄球菌、大肠杆菌O157:H7和福氏志贺氏菌,利用表面增强拉曼(SERS)光谱技术,以原位包覆纳米银为基底,获得5种细菌的SERS谱。结果表明:在400~2 000 cm^(-1)光谱内,布鲁氏菌S2株有10处明显的拉曼谱峰,鼠伤寒沙门氏菌有9处明显的拉曼谱峰,金黄色葡萄球菌有5处明显的拉曼谱峰,大肠杆菌O157:H7有9处明显的拉曼谱峰,福氏志贺氏菌有7处明显的拉曼谱峰。5种食源性致病菌的拉曼谱峰位置以及强度区别明显。利用SERS技术可以实现在10 min内识别5种食源性致病菌,达到增强细菌拉曼信号、快速检测食源性致病菌的目的。Surface enhanced Raman spectroscopy（ SERS） was applied to quickly detect Brucellasuis vaccine strain 2（ B. suis S2）,Salmonella typhimurium（ S. typhimurium）,Staphylococcus aureus（ S.aureus）,Escherichia coli O157： H7（ E. coli O157： H7） and Shigellaflexneri（ S. flexneri）,respectively. According to the enhancement effect of SERS,the method of in Situ Coating with Ag Nanoparticles was used to enhance the bacteria Raman signal.Within the range of 400-2000 cm-1,B.suis S2 had 10 distinct Raman peaks,S. typhimurium had 9 distinct Raman peaks,S. aureus had 5 distinct Raman peaks,E. coli O157： H7 had 9 distinct Raman peaks,and S. flexneri had 7 distinct Raman peaks. And the position and intensity of Raman spectra of the five foodborne bacteria were different. We can rapidly detect five foodborne pathogens within 10 minutes using SERS technique,which provides a basis for clinical diagnosis,and the purpose of detecting bacteria by SERS was achieved quickly.

关 键 词：表面增强拉曼 食源性致病菌 布鲁氏菌S2株 鼠伤寒沙门氏菌 金黄色葡萄球菌 大肠杆菌O157:H7 福氏志贺氏菌 

分 类 号：R155.3[医药卫生—营养与食品卫生学] TS201.3[医药卫生—公共卫生与预防医学]