纳豆激酶分批补料发酵的研究  

Production of nattokinase by fed-batch fermentation

作　　者：吴燕[1,2] 梁向峰 刘会洲 李英波[2] 肖建辉[1] WU Yan;LIANG Xiang-feng;LIU Hui-zhou;LI Ying-bo;XIAO Jian -hui(Institute of Medicinal Biotechnology, Affiliated Hospital of Zunyi Medical University, Zunyi 563003, China;Chinese Academy of Sciences, Institute of Process Engineering, Key Laboratory of Green Process and Engineering, Beijing 100190, China;Chinese Academy of Sciences, Qingdao Institute of Bioenergy and BioprocessTechnology, Key Laboratory of Bio - based Material, Qingdao 266101, China)

机构地区：[1]遵义医学院附属医院,医药生物技术研究所,贵州遵义563003 [2]中国科学院过程工程研究所,中国科学院绿色过程与工程重点实验室,北京100190 [3]中国科学院青岛生物能源与过程研究所,中国科学院生物基材料重点实验室,山东青岛266101

出　　处：《食品与发酵工业》2018年第1期126-132,共7页Food and Fermentation Industries

摘　　要：在摇瓶和发酵罐上研究了分批补料发酵对枯草芽孢杆菌LSSE-22发酵生产纳豆激酶的影响。通过摇瓶分批发酵,确定最优碳源和氮源分别为葡萄糖和大豆蛋白胨。在优化初始葡萄糖和大豆蛋白胨浓度的基础上,进一步研究了补料底物、补料方式和补料时间对产酶的影响。结果表明,采用分批补糖发酵工艺,纳豆激酶产量可达到1 437.34 IU/m L,比分批培养提高了21.38%。在7.5 L发酵罐上进行分批补料发酵放大实验,纳豆激酶产量可达2 046.47 IU/m L,明显优于分批培养。This paper studied the effect of nattokinase production from Bacillus subtilis LSSE-22 in shake flask and bioreactor. Glucose and soy peptone were identified as the optimal carbon source and nitrogen source through batch fermentation in shake flask, thus were used in this study. Furthermore, the effect of feeding substrate, method and time on the nattokinase production was also studied at optimized initial glucose and soy peptone concentration. The results showed that feeding method produced 1 437.34 IU/mL of nattokinase, which was 21.38% higher than that by batch culturing method. In the 7.5 L bioreactor, the maximum nattokinase concentration reached 2 046.47 IU/mL.

关 键 词：纳豆激酶 枯草芽孢杆菌 分批补料发酵 分批发酵 

分 类 号：TQ925[轻工技术与工程—发酵工程]