基于竞争性等位基因特异性PCR技术对啤酒花进行纯度检测  

Based on Kompetitive allele specific PCR methodology for the purity detection of Humulus iupulus varieties

作　　者：蒋培基 王德良[2] 江伟[2] 宋涛[2] 李涛[2] 蒲彪[1] 栾春光[2] JIANG Pei-ji;WANG De-liang;JIANG Wei;Song Tao;Tao;PU Biao;LUAN Chun-guang(College of Food Science, Sichuan Agricultural University, Ya＇an 625014, China;China National Research Institute of Food and Fermentation Industries, Beijing 10015, China)

机构地区：[1]四川农业大学食品学院,四川雅安625014 [2]中国食品发酵工业研究院,北京100015

出　　处：《食品与发酵工业》2018年第1期211-216,共6页Food and Fermentation Industries

基　　金：中国食品发酵工业研究院科研发展基金(2017-酿酒-505)

摘　　要：基于已知数据库中啤酒花的基因组信息和测序信息设计引物,筛选出了可以区分7个啤酒花品种的8个多态性良好的单核苷酸多态性(single nucleotide polymorphisms,SNP)位点。并基于竞争性等位基因特异性PCR(Kompetitive allele specific PCR,KASP)技术,利用筛选到的特异SNP标记对8份预混样品与2份企业送检样品进行了检测。结果表明,该技术能对混杂程度低至5%的啤酒花样品进行有效鉴定,可以满足企业对实际啤酒花样品进行检测评价的要求。研究结果完善了啤酒花的SNP数据库,为啤酒企业对于原料质量的把控提供有效的技术手段。Hops is an important raw material in beer brewing industries. However, the adulteration of the com- mercial hops has seriously impact the stability of beer quality. Therefore, it is necessary to develop a robust purity de- tection method for commercial hops. Based on the known genomic database online and sequencing information of this study, primers were designed to amplify the target SNP markers. The resulting 8 polymorphic SNP loci that could dis- tinguish 7 hops cuhivars were screened and confirmed. Using Kompetitive allele specific PCR （Kompetitive Allele Specific PCR, KASP） technology based on SNP（Single Nucleotide Polymorphisms） , 2 corporate samples and 8 pre- mixed samples were well characterized. The results showed that adulterated hops lower than 5% could be identified u- sing this method. This method can be used to monitor the quality of raw materials in beer and other relative food in- dustries in the future.

关 键 词：啤酒花 单核苷酸多态性 基因特异性PCR 真实性检测 纯度检测 

分 类 号：TS262.5[轻工技术与工程—发酵工程]