干酪乳杆菌的筛选及其在活性乳酸菌饮料中的应用  

Screening of Lactobacillus casei and application in active lactic bacteria beverage

作　　者：杨子燕 赵世伟 马万平 赵旭燕 尹柯茹 陈敏 杨子彪 

机构地区：[1]云南皇氏来思尔乳业有限公司,云南大理671003

出　　处：《食品工业科技》2018年第3期106-111,116,共7页Science and Technology of Food Industry

基　　金：云南省优质益生菌菌种选育及高原特色乳制品产业化开发科技示范(2014ZA001)

摘　　要：目的:为了筛选应用于活性乳酸菌饮料生产的优质益生菌。方法:以云南省各地区发酵样品中分离出的120株干酪乳杆菌为研究对象,筛选具有人工胃液、人工肠液、胆盐耐受性及对肠道致病菌有抑制作用的菌株,同时将筛选出的菌株用于褐色活性乳酸菌饮料的生产发酵实验。结果:筛选出四株干酪乳杆菌L.casei1、L.casei2、L.casei3、L.casei5,有较强的耐酸、耐胆盐及耐受人工消化液的能力;能抑制肠道致病菌:大肠杆菌、福氏志贺氏菌、肠炎沙门氏菌以及革兰氏阳性的金黄色葡萄球菌,所以具有调节人体肠道菌群平衡的益生潜力。在进行200 L发酵罐的生产摸拟实验中干酪乳杆菌L.casei1、L.casei2、L.casei3、L.casei5四株菌的褐色活性乳酸菌饮料成品在口感、稳定性及均能满足生产应用要求,具有很好的研究及应用价值。Objectives： For selecting finest probiotics can be applied to lactobacillus beverages.Methods： 120 strains of Lactobacillus casei isolated from fermented foods of Yunnan provice were studied,for obtaining Lactobacillus caseis that can adapt to simulated digestive tract environment,inhabite intestinal pathogenic bacterias. Then,they are used in fermentation experiment of brown production of active lactobacillus drink.Conclusion： we screen out 4 strains of Lactobacillus casei including L.casei1,L.casei2,L.casei3,L.casei5 with strong tolerance to simulated gastric fluid,man-made digestive juice and bile salt,the four strains of Lactobacillus casei have antagonistic activity against the intestinal pathogenic bacterias,sothey have probiotic potential adjusting the balance of intestinal flora. L.casei1,L.casei2,L.casei3,L.casei5 can satisfy the production requirements through fermentation experiments of brown production of active lactobacillus drink.

关 键 词：干酪乳杆菌 益生潜力 抑菌活性 褐色活性乳酸菌饮料 

分 类 号：TS201.3[轻工技术与工程—食品科学]