响应面法优化金针菇液体发酵培养基  

Optimization of Liquid Fermentation Medium of Flammulina velutipes by Response Surface Methodology

作　　者：陈敏[1,2] 洪家丽 雷雅婷 陈岚彬 陈永芳 万旭志 龚世禹 刘艺琳 余晓丹 曾峰 刘斌[1,2,3] CHENMin;HONG Jiali;LEI Yating;CHEN Lanbin;CHEN Yongfang;WAN Xuzhi;GONG Shiyu;LTU Yilin;YU Xiaodan;ZENG Feng;UU Bin(College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;National Engineering Research Center of JUNCAO Technology, Fuzhou, Fujian 350002, China;College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China)

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]国家菌草工程技术研究中心,福建福州350002 [3]福建农林大学生命科学学院,福建福州350002

出　　处：《农产品加工》2018年第2期1-4,共4页Farm Products Processing

基　　金：国家科技支撑计划子课题(2014BAD15B01);福建省科技重大专项(2014NZ2002-1);福建省科技厅区域发展项目(2016N3006)

摘　　要：采用四因素三水平的Box-Behnken中心组合设计进行响应面法分析,考查葡萄糖、蛋白胨、KH_2PO_4和果蔬汁添加量对金针菇液体发酵菌丝生物量的影响。结果表明,在金针菇深层液体发酵试验中,最优培养基质量分数为葡萄糖2.15%,蛋白胨0.38%,KH_2PO_40.27%,果蔬汁12.90%;在优化培养基组成的条件下,预测金针菇深层发酵菌丝体产量最大值达21.51 mg/m L,与实际测量值21.46 mg/m L在误差范围内。The effects of glucose, peptone, KH2 PO4, fruit and vegetable juice on the biomass of Flammulina velutipes were studied by response surface methodology using Box-Behnken center combination design with 4 factors and 3 levels. The results showed that the optimum medium mass fraction was glucose 2.15% , peptone 0.38% , KH2 PO4 0.27% , fruit and vegetable juice 12.90%. Under the condition of optimizing the composition of the medium, the maximum yield of mycelium of Flammuli-na velutipes was 21.51 mg/mL and the actual measured value was 21.46 mg/mL.

关 键 词：响应面 金针菇 果蔬汁 菌丝体 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]