谷氨酸发酵过程膜偶联间歇透析发酵工艺研究  

Study on Membrane Coupled Intermittent Dialysis Fermentation Process for Glutamic Acid Fermentation

作　　者：户红通 徐达 徐庆阳 刘子强[1] 蔡萌萌[1] 陈宁[2,3,1] 

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]代谢控制发酵技术国家地方联合工程实验室,天津300457 [3]天津市氨基酸高效绿色制造工程实验室,天津300457

出　　处：《食品与发酵科技》2018年第1期9-13,23,共6页Food and Fermentation Science & Technology

基　　金：2016绿色制造系统集成子课题(财建[2016]1008号-37-2)

摘　　要：采用膜偶联间歇透析发酵工艺,解除了胞内谷氨酸的反馈调节作用及有毒害副产物的抑制作用,促使谷氨酸代谢流增加,产酸速率提高。结果表明:在30L发酵罐上普通发酵单罐产酸2.688kg,透析发酵单罐产酸5.232kg,单罐谷氨酸产量提高了94.64%,产酸周期延长了16h左右;普通发酵的总糖酸转化率为66.3%,而透析发酵的总糖酸转化率为69.8%,提高了3.5%;主要代谢副产物乳酸的代谢流平均降低了28.1%,丙氨酸的代谢流平均降低了20.0%,而目的产物谷氨酸的代谢流量由73.47提高至76.45,提高了4.1%。另外,发酵液的质量同时得到了改善,有利于发酵过程控制及谷氨酸分离提取。Membrane coupled intermittent dialysis fermentation process was used, which eliminated the feedback regulation of intracellular glutamate and the inhibition of toxic byproducts, increasing the flow of glutamate metabolism and improving acid production rate. The result showed that the yield of single tank of ordinary fermen- tation in 30L fermentation tank was 2.688kg and single tank of dialysis fermentation was 5.232kg, the yield of glutamic acid in single tank increased by 94.64%, the acid production time was also extended for about 16 hours; the total sugar and acid conversion rate of common fermentation was 66.3% and the total sugar and acid conver- sion rate of dialysis fermentation was 69.8%, improved by 5.3%. The metabolic flow of lactic acid, an important metabolic byproduct, was reduced by an average of 28.1%, the metabolic flow of alanine was reduced by an av- erage of 20%, and the metabolic flux of glutamic acid increased from 73.47 to 76.45, improved by 4.1%. In ad- dition, The quality of the fermented liquid was also improved simultaneously, which also provided advantages for fermentation process control and glutamic acid isolation and extraction.

关 键 词：谷氨酸 膜偶联间歇透析发酵 糖酸转化率 副产物 

分 类 号：TQ922.1[轻工技术与工程—发酵工程]