高效液相色谱法测定乳及乳制品中泛酸分析方法的改进  

Optimization the method for determination of pantothenic acid in milk and dairy product by high performance liquid chromatography

作　　者：刘琳[1] 刘岩 段小娟 翟丽娜[1] 郝鹏飞[1] 张丽 吕宁[1] 徐琴[1] 

机构地区：[1]山东出入境检验检疫局检验检疫技术中心,青岛266002 [2]青岛大学化学化工学院,青岛266071 [3]青岛出入境检验检疫局,青岛266002 [4]临沂出入境检验检疫局综合技术服务中心,临沂276034

出　　处：《食品安全质量检测学报》2017年第11期4190-4194,共5页Journal of Food Safety and Quality

摘　　要：目的改进和优化了高效液相色谱法(high performance liquid chromatography,HPLC)检测乳及乳制品中泛酸的分析方法。方法对测定泛酸的GB 5413.17-2010《食品安全国家标准婴幼儿食品和乳品中泛酸的测定》的HPLC分析方法的流动相进行优化,然后对优化后的方法进行方法学的验证。结果选择0.05%磷酸水溶液+乙腈作为流动相,色谱图上的目标峰得到完全分离,分离效果好。线性方程为Y=1.3735X+23.0911,r^2=0.99996。方法回收率为83.20%~110.75%,相对标准偏差在3.43%~5.96%之间,具有较好的精确度和重现性。结论该方法快速、简便、准确,适用性好,有效地去除了干扰物质,克服了高效液相色谱检测中泛酸背景值高、干扰多的问题。Objective To optimize the method for determination of pantothenic acid in milk and dairy product by high performance liquid chromatography（HPLC）. Methods According to the first method of GB 5413.17-2010 National food safety standard-Detemination of pantothenic acid in foods for infants and young children, milk and milk products, the mobile phase was optimized in pantothenic acid analysis by HPLC, and the optimized method was verified by methodology. Results The 0.05% phosphoric acid＋acetonitrile were chosen as mobile phase, the target peaks on the chromatogram were completely separated and the separation effect was good. The linear equation was Y=1.3735 X＋23.0911 with r^2=0.99996. The recoveries were 83.20%-110.75% and relative standard deviations were 3.43%-5.96%. Conclusion This method is rapid, simple, accurate and suitable for the removal of interfering substances. It overcomes the problem of high background value and interference in HPLC detection.

关 键 词：乳及乳制品 泛酸 高效液相色谱法 

分 类 号：O657.72[理学—分析化学] TS252.7[理学—化学]