乳酸菌抑制病原菌对奶牛乳腺上皮细胞的粘附和侵入作用  

Inhibition of Pathogens Adhesion and Internalization to bMEC by Lactic Acid Bacteria

作　　者：许女[1,2] 史改玲[1] 张浩[1] 杜立红[1] 王俊东[2] 

机构地区：[1]山西农业大学食品科学与工程学院山西农业大学,山西太谷030801 [2]山西省生态畜牧与环境兽医学重点实验室山西农业大学,山西太谷030801

出　　处：《中国食品学报》2017年第11期20-27,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金："十二五"国家科技支撑计划项目(2012BAD 12B 06)

摘　　要：考察了分离自健康牛乳管中的乳酸乳球菌CM2和来源于奶牛肠道中的干酪乳杆菌CI2,对奶牛乳腺炎病原菌金黄色葡萄球菌SMS81和大肠杆菌SME177的粘附侵入乳腺上皮细胞的抑制作用;研究了菌体细胞侵入乳腺上皮细胞后相关抗菌肽表达的情况。结果表明:两株乳酸菌CM2和CI2具有显著抑制病原菌粘附和侵入b MEC的能力,并且具有一定的浓度依赖性;加热灭活处理的乳酸菌仍然具有抑制病原菌粘附b MEC的能力,但抑制侵入的能力严格依赖于菌体细胞的活性;乳酸菌细胞培养上清液还可以降低病原菌对b MEC细胞的粘附能力,这可能与乳酸菌产生细菌素有关。实时荧光定量PCR结果显示:CM2菌株显著提高了金黄色葡萄球菌SMS81和大肠杆菌SME177侵入b MEC后抗菌肽TAP和BNBD10基因的表达水平,推测可能与乳酸菌抑制病原菌侵入内化牛乳腺上皮细胞有关。本研究成果为奶牛乳腺炎天然、安全、无污染的有效治疗提供了新的开发前景。In this study, we tested the ability of Lactococcus lactis CM2（isolated from mammary gland of healthy cows） and Lactobacillus casei CI2（isolated from intestinal tract of cows） to prevent invasion of b MEC by two bovine mastitis pathogenic strains, S. aureus SMS81 and E. coli SME177. The results showed that the two lactic acid bacteria strains could inhibit the adhesion and internalization of pathogens in a concentration-dependent manner. Moreover, the inhibition of pathogens adhesion was maintained with heat-killed lactic acid bacterias, whereas live cells was required to prevent pathogens internalization. Also, the culture supernatant of lactic acid bacteria could reduce the adhesion ability of pathogenic cells to b MEC, and this may be related to bacteriocins produced by the strains. Additionally, CM2 significantly up-regulate the antimicrobial peptide TAP and BNBD10 m RNA expression, which maybe involved in the inhibition of pathogens invasion and internalization into b MEC by lactic acid bacteria. Our study will opens avenues for the development of a novel natural, safe and efficient control strategies against bovine mastitis.

关 键 词：乳酸菌 乳腺上皮细胞 粘附 侵入 

分 类 号：S858.23[农业科学—临床兽医学]