清远市健康人群全血8种矿物质含量分布调查  

Distribution of 8 mineral contents in whole blood of healthy individuals in Qingyuan

作　　者：苏祖俭 姚敬[1] 李敏[1] 胡曙光[1] 蔡文华[1] 陈明[1] 范建彬 黄泓耀 黄伟雄[1] 

机构地区：[1]广东省疾病预防控制中心,广东广州511430 [2]中山大学公共卫生学院

出　　处：《华南预防医学》2018年第1期29-33,共5页South China Journal of Preventive Medicine

摘　　要：目的建立全血钠、镁、钾、钙、锰、铁、铜、锌元素的测定方法,并对广东省清远市健康人群全血8种矿物质含量进行调查,分析其含量特点和性别、年龄差异。方法采用分层多级随机抽样方法抽取清远地区8个县(市、区)的25~70岁健康居民作为研究对象。建立微波消解-电感耦合等离子体质谱方法,并采用所建立的方法检测研究对象全血8种矿物质的含量。采用Shaprio-Wilk法对所得数据进行正态性检验,并进行偏度和峰度分析;采用Wilcoxon符号秩检验分析不同性别、年龄健康成人全血中矿物质含量差异。结果本研究所建立的方法各元素线性关系均良好(相关系数>0.999 1),方法的检出限为0.668μg/L^0.013 8 mg/L,相对标准偏差为0.54%~6.98%。研究对象全血8种矿物质的含量均不服从正态分布,其中钠、镁、钾、钙、铁、铜呈负偏态分布,锰、锌呈正偏态分布;钠、镁、钾、钙、锰、铜呈尖峰分布,铁、锌呈扁平分布。不同性别健康成人全血8种矿物质含量差异均有统计学意义(均P<0.05)。不同年龄组健康成人中除25~29与30~39岁年龄组人群的血钾和血铁、30~39与40~49岁年龄组人群的血铜、40~49与50~59岁年龄组人群的血镁外,其余相邻2个年龄组成年人全血8种矿物质含量差异均无统计学意义(均P>0.05)。结论所建立的方法检出限低,准确度高,样品处理、测定迅速,能满足全血中矿物质的测定。清远市健康人群全血8种矿物质含量均不服从正态分布,且具有性别差异。Objective To establish a method for the determination of Na, Mg, K, Ca, Mn, Fe, Cu and Zn in whole blood and analyze the mineral contents in whole blood of healthy induvidials in Qingyuan City, Guangdong Province. Methods A stratified multistage random sampling method was used to select healthy residents aged 25 -70 years from 8 counties （cities, districts） in Qingyuan. A method by microwave digestion and inductively coupled plasma mass spectrometry （ICP-MS） was established and used to detect the contents of 8 minerals in whole blood of the subjects investigated. The data were tested for nor- mality using the Shaprio-Wilk method, then the skewness and kurtosis were also analyzed. Wilcoxon signed rank test used to analyse the difference of mineral contents in whole blood of healthy adults with different genders. Results The linear relationship of each mineral established in this study was good （ correlation coefficient 〉0.999 1 ）. The detection limit of the method was between 0. 668 μg/L and 0. 013 8 rag/L, and the relative standard deviation, from O. 54% to 6.98%. The contents of 8 minerals in whole blood were not normally distributed. The distributions were negatively skewed for Na, Mg, K, Ca, Fe, and Cu, while skewed for Mn and Zn. Na, Mg, K, Ca, Mn, Cu were in peak distribution while Fe and Zn were fiat distribution. There were significant differences in 8 mineral contents in whole blood of healthy adults with different genders （ all P 〈 0.05 ）. There were no significant differences in 8 mineral contents in whole blood between 2 age groups （ all P 〉 0.05 ） except for blood K and blood Fe in groups aged 25 - 29 and 30 - 39 years, blood Cu in groups aged 30 - 39 and 40 - 49 years and blood Mg in groups aged 40 - 49 and 50 - 59 years. Conclusion The established method has low detection limit, high accuracy, rapid sample processing and rapid determination, and can meet the determination of minerals in whole blood. The contents of 8 minerals in whole blood of healthy individuals in Qingyu

关 键 词：健康人群 血液 矿物质 

分 类 号：R151[医药卫生—营养与食品卫生学]