槐角多糖表征及抗氧化吸湿保湿性能研究  

Structural characterization and antioxiant,moisture-absording and moisture-retention properties of polysaccharides of Sophora japonica L.

作　　者：李彩霞[1,2] 郑雪 高海宁 张勇[1,2] LI Cai-xia1,2 ,ZHENG Xue1, GAO Hai-ning1,2, ZHANG gong1,2(1. College of Agriculture and Biotechnology, Hexi University, Zhangye, Gansu 734000, China ; 2. Key Laboratory of Hexi Corridor Resources Utilization of Gansu Universities, Zhangye , Gansu 734000, Chin)

机构地区：[1]河西学院农业与生物技术学院,甘肃张掖734000 [2]甘肃省高校河西走廊特色资源利用省级重点实验室,甘肃张掖734000

出　　处：《食品与机械》2017年第12期17-22,共6页Food and Machinery

基　　金：国家中医药管理局2012年中医药行业科研专项(编号:201207002)

摘　　要：采用双酶法提取槐角果皮和种子多糖,通过红外光谱对多糖进行表征,并对多糖的抗氧化能力、吸湿和保湿性能进行评价。结果表明:双酶法从槐角果皮和种子中提取的多糖得率分别为24.7%,11.6%。红外光谱结果显示:槐角果皮和种子多糖均具有多糖的特征吸收峰,种子多糖具有β-吡喃糖苷键,果皮多糖具有β-型和α-型2种糖苷键。自由基清除试验显示,槐角果皮和种子多糖具有一定的自由基清除能力,在一定浓度范围内,种子多糖的清除自由基能力优于果皮多糖,且二者的清除能力与其浓度呈正相关;与阳性对照VC相比,2种多糖对ABTS、DPPH自由基的清除作用较弱,但种子多糖对超氧阴离子的清除作用优于VC;与阳性对照EDTA相比,2种多糖对亚铁离子的螯合能力较弱。在相同条件下,2种多糖的吸湿性与壳聚糖一致但低于甘油;二者保湿性相当,均低于甘油,但高于壳聚糖。说明槐角不同部位含有丰富的多糖,有较好的自由基清除活性和保湿性,具有开发利用价值。The polysaccharides from the seeds and peel of Soppora japonica g. were extracted respectively by double enzymes, The basic structure of polysaccharides were identified by using infrared spectroscopy,and antioxidant activity, moisture absorption and retention capacities of polysaccharides were evaluated. The results indi cared that the poysaccharides extraction yield of Sopkora japonica L. seeds and peel were 11.6% and 24.7% respectively. The IR spec trum indicated peel and seed polysaccharides had typical characteristic absorption peaks of polysaccharides and pyran ring structure, seed polysaccharides contained a-glycosidic bonds, peel polysaccharides contained a and βglycosidic bonds. The radical scavenging experiment showed that two different polysaccharides of Sophora japonica L. had a certain radical scavenging capacity. Within a certain concentration range, the radical scavenging activity of seeds polysaccharides was better than that of peel polysaccharides, and had the positive correlations with concentration of polysaccharides. Compared with the positive control vitamin C（Vc）, the ABTS and DPPH radical scavenging ability of two polysaccharides were weak, but the scavenging effect of seeds polysaceharide on superoxide anion was better than that of Vc. Compared with the positive control EDTA, determination of two polysaccharides on ferrous ion chelating ability were weak. In the same conditions, moisture absorption capacities of two polysaccharides of sophora fructus were close to that of chitosan but lower than that of glycerin. Moisture retention capacities of two poly- saccharides of sophora fructus were equivalent, lower than glycerin, but higher than chitosan. These results demonstrated that polysac- charides of Sophora japonica L. had good radical scavenging activity and moisture retention capacity which had the development and utili zation value.

关 键 词：槐角 多糖 抗氧化活性 吸湿保湿活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]