诱变蜡状芽孢杆菌产磷脂酶C的研究  

Studies on Mutagenesis Bacillus cereus Producing Phospholipase C

作　　者：宋玉卿[1] 王彤[2] 李丹[2] 闫世长 于殿宇[2] 张康逸[3] 王俊国[1] Song Yuqing1, Wang Tong2, Li Dan2, Yan Shizhang2, Yu Dianyu2, Zhang Kangyi3, Wang Junguo1(1Jinlin Business and Technology College, Changchun 130507;2College of Food Science and Technology, Northeast Agricultural University, Harbin 150030;3Henan Academy of Agricultural Sciences, Institute of Agricultural Products Processing, Zhengzhou 45000)

机构地区：[1]吉林工商学院,长春130507 [2]东北农业大学食品学院,哈尔滨150030 [3]河南省农科院农副产品加工研究所,郑州450002

出　　处：《中国食品学报》2018年第3期141-149,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家十三五攻关课题(2016YFD0401402);国家自然科学基金项目(31571880);黑龙江省自然科学基金(C2017019)

摘　　要：采用3种诱变方法处理出发菌株蜡状芽孢杆菌,得到1株高产磷脂酶C菌株蜡状芽孢杆菌-3。选用沉淀圈直径及活菌数作为参考指标,响应面法优化蜡状芽孢杆菌-3菌株培养条件,结果:接种量2.5%,转速160 r/min,发酵温度30℃,发酵时间18.5 h。此条件下,沉淀圈直径最大,磷脂酶C活力达26.23 U/m L。对粗酶液进行分离纯化,利用SDS-聚丙烯酰胺凝胶电泳测定纯化后的磷脂酶C分子质量,其分子质量在29~34 ku之间。The departure strains of Bacillus cereus are handling by three kinds of mutagenesis, and get a phospholipase C high-yield strains of B.cereus-3. Selection of the precipitation ring diameter and the number of viable counts as a reference. By response surface to optimize the fermentation conditions of the strain B. cereus-3, the result for that： the inoculum size is 2.5%, speed is 160 r/min, fermentation temperature is 30.5 °C and the fermentation time is18.5 h. At the time, the precipitation ring diameter is maximum, and phospholipase C activity is 26.3 U/m L. The crude enzyme solution were isolated and purified, and used SDS-PAGE method to measure the molecular weight of the purified phospholipase C, molecular weight between about 29-34 ku.

关 键 词：蜡状芽孢杆菌 诱变 磷脂酶C 酶活力 

分 类 号：TQ925[轻工技术与工程—发酵工程]