山河陈醋大曲淀粉酶系分析  

The Analysis of Amylase Series in Daqu of Shanhe Mature Vinegar

作　　者：李中正 曹彦军 吕玉柱 郝林[1] LI Zhong-zheng;CAO Yan-jun;LV Yu-zhu;HAO Lin(College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, China;Shanhe Vinegar Co. , Ltd. , Heshun 032700, China)

机构地区：[1]山西农业大学食品科学与工程学院,山西太谷030801 [2]山河醋业有限公司,山西和顺032700

出　　处：《中国调味品》2018年第4期45-48,共4页China Condiment

基　　金：校企横向联合项目(2016HX17)

摘　　要：从山河陈醋大曲中用透明圈法筛选得到具有较高淀粉酶活力的7株细菌和5株霉菌,经鉴定,细菌均为芽孢杆菌属(Bacillus),编号:X17,X4,X1,X7,X20,X5,X8,霉菌中编号M4,M13,M17的菌株为曲霉属(Eurotium),编号M7的菌株为犁头霉属(Absidia),编号M1的菌株为毛霉属(Mucor)。分别接入麸皮培养基进行纯种发酵,对淀粉酶系分析。结果:山河大曲的α-淀粉酶活力为1.540U/g,β-淀粉酶活力为215.622U/g,葡萄糖淀粉酶活力为816.815U/g,淀粉脱支酶活力为0.073U/g;细菌中X5的α-淀粉酶活力最高为4.006U/g,X7的β-淀粉酶活力最高,为48.096U/g,X1的葡萄糖淀粉酶活力最高,为792.824U/g,X1的淀粉脱支酶活力最高,为0.064U/g;霉菌中M4的α-淀粉酶活力最高,为6.238U/g;M13的β-淀粉酶活力、葡萄糖淀粉酶活力、淀粉脱支酶活力最高,分别为234.664,1204.368,0.101U/g。Gain seven strains of bacteria and five strains of mould which have high amylase activity from Daqu of Shanhe mature vinegar by screening of transparent circle method.According to evaluation,the bacteria are all Bacillus,their serial number is X17,X4,X1,X7,X20,X5,X8.The strains M4,M13,M17 are Eurotium,the strain M7 is Absdia,the strain M1 is Mucor.Respectively attaching bran medium for pure fermenting and analysis of amylase series.The results are：the activity ofα-amylase in Daqu is1.540 U/g,the activity ofβ-amylase is 215.622 U/g,the activity of glucose amylase is 816.815 U/g,the activity of starch debranching enzyme is 0.073 U/g;the activity ofα-amylase of X5 is the highest in bacteria,which is 4.006 U/g,the activity ofβ-amylase of X7 is the highest,which is 48.096 U/g,the activity of glucose amylase of X1 is the highest,which is 792.824 U/g,the activity of starch debranching enzyme of X1 is highest,which is 0.064 U/g,the activity ofα-amylase of M4 in mould is the highest,which is 6.238 U/g;the activities ofβ-amylase,glucose amylase,starch debranching enzyme of M13 are the highest,which is 234.664,1204.368,0.101 U/g respectively.

关 键 词：Α-淀粉酶 Β-淀粉酶 葡糖糖淀粉酶 淀粉脱支酶 

分 类 号：TS264.22[轻工技术与工程—发酵工程]