水浴消解与传统工艺制备北部湾南美白对虾甲壳素研究  

Study on Preparation of Chitin from Penaeus vannamei by Water Bath Digestion and Traditional Technology

作　　者：蓝尉冰[1,2,3] 王帅静 韩鑫 肖金亮[1] LAN Wei-bing;WANG Shuai-jing;HAN Xin;XIAO Jin-liang(Qinzhou University, Qinzhou 535099, China;Key Laboratory of Development and High-value Utilization of Beibu Gulf Seafood Resources in Guangxi Colleges and Universities, Qinzhou 535099, China;Guangxi University, Nanning 530004, China;Wuhan University of Technology, Wuhan 430063, China)

机构地区：[1]钦州学院,广西钦州535099 [2]广西高校北部湾特色海产品资源开发与高值化利用重点实验室(钦州学院),广西钦州535099 [3]广西大学,南宁530004 [4]武汉理工大学,武汉430063

出　　处：《中国调味品》2018年第4期59-63,共5页China Condiment

基　　金：广西高校北部湾特色海产品资源开发与高值化利用重点实验室(钦州学院)(2016ZB05);2016年度广西高校中青年教师基础能力提升项目(KY2016LX423);钦州学院青年科研基金项目(2013XJKY-48Q);广西北部湾海洋生物多样性养护重点实验室(钦州学院)自主项目(2015ZC06)

摘　　要：实验以广西北部湾地区南美白对虾虾头、虾壳为原料,采用水浴消解技术辅助制备甲壳素,通过单因素和正交试验,探讨最佳工艺条件。结果表明:脱蛋白率最佳条件为:NaOH浓度3%,时间1.5h,温度80℃,料液比1∶20(g/mL),时间、NaOH浓度对脱蛋白率符合Gauss函数模型,所得拟合曲线方程系数分别为y0=50.53232,xc=1.49095,w=1.34464,A=20.99076,R2=0.98及y0=38.94297,xc=3.7871,w=3.17071,A=111.26518,R2=0.96;经3%HCl 60℃水浴1.0h后用10%过氧化氢溶液80℃水浴脱色2.0h。结果表明:水浴消解技术甲壳素得率为23.94%,而传统工艺甲壳素得率为14.63%,水浴消解技术明显优于传统工艺。In this experiment,the shrimp head and shell in Guangxi Beibu Gulf area are used as raw materials,and chitin is prepared by water bath digestion technology.The optimum process conditions are investigated by single factor and orthogonal test.The results show that the optimal conditions of protein removal rate are the concentration of NaOH of 3%,time of 1.5 h,temperature of 80℃,ratio of material to liquid of 1∶20（g/mL）.Time and concentration of NaOH on protein removal rate are consistent with the Gauss function model,the coefficients of fitting curve equation are y0=50.53232,xc=1.49095,w=1.34464,A=20.99076,R2=0.98 and y0=38.94297,xc=3.7871,w=3.17071,A=111.26518,R2=0.96.The protein removal products are treated with 3% HCl in 60℃ water bath for 1 hand 10% hydrogen peroxide solution at 80 ℃for 2 h.The results show that the yield rate of the product by the water bath digestion technology is 23.94%,while the yield rate of the product is by the traditional process 14.63%.Water bath digestion technique is superior to the traditional process.

关 键 词：南美白对虾 虾壳 甲壳素 传统工艺 水浴消解 

分 类 号：TS201.1[轻工技术与工程—食品科学]