海黍子褐藻糖胶的分离及抗氧化与保湿活性  

Separation of Different Molecular Weight of Fucoidan from Sargassum kjellmanianum and Their Antioxidant and Moisturizing Activities

作　　者：陶传云 周革非[1] 马伟伟[1] TAO Chuanyun;ZHOU Gefei;MA Weiwei(Life Sciences College of Yantai University （Yantai 264005)

机构地区：[1]烟台大学生命科学学院,烟台264005

出　　处：《食品工业》2018年第4期225-229,共5页The Food Industry

基　　金：山东省自然科学基金项目(ZR2014DL011);国家自然科学基金(31572622)

摘　　要：采用酸提法从海黍子中提取得到海黍子粗多糖,经60%醇沉得褐藻糖胶粗多糖,经去褐藻酸、蛋白质及小分子等杂质后,通过epherose-6B分级纯化得到三个组分(SKP1,SKP2和SKP3),其相对分子质量分别为837,271和610k Da。对其结构组成及抗氧化与保湿活性进行测定,结果表明:三种多糖均是硫酸化多糖,均主要由不同比例的岩藻糖、半乳糖和甘露糖组成,另含有少量的阿拉伯糖、木糖和鼠李糖等;三种多糖还原能力与VC接近,且均表现出明显的清除自由基活性,且活性大小与相对分子质量和糖醛酸含量成反比,与硫酸基含量成正比;SKP1和SKP2的吸湿活性分别高出透明质酸钠22.32%和2.38%,三种褐藻糖胶的保湿率分别比甘油大35.88%,27.96%和22.31%,且随相对分子质量越大,保湿与吸湿活性越大。Polysaccharide was extracted with acid, and fucoidan was got using alcohol precipitation method at 60% ethanol from Sargassum kjellmanianum. Three fractions（SKP1, SKP2 and SKP3） were got through removing alginate, protein and small molecular impurities, then classified by sepharose-6 B, whose molecular weights were 837, 271 and 610 k Da, respectively. Their composition, structure, anti-oxidation and moisturizing activity were measured, and the results showed that the three samples were all sulfated polysaccharides. They were composed mainly by a different proportion of fucose, galactose and mannose, also a small amount of arabinose, xylose and rhamnose. Their reducing capacities were close to VC and all of them showed up significant free radical scavenging activity, meanwhile, the activity was negative correlative with molecular weight and uronic acid content while having a positively correlation with sulfate content. Hygroscopicities of SKP1 and SKP2 were 22.32% and 2.38%, higher than hyaluronate. Moisturizing rates of the three fucoidan samples were 35.88%, 27.96% and 22.31%, higher than glycerol. Moisturizing and moisture activity were positively correlation with molecular weight.

关 键 词：海黍子 褐藻糖胶 抗氧化 保湿 吸湿 

分 类 号：TS201.2[轻工技术与工程—食品科学]