下喷式液泵型生物反应器与通用发酵罐K_Lα对比及应用  

KLacomparison and application of reverser jet loop reactor and universal fermenter

作　　者：何欢 郑哲堂 符爽 袁淼洪 张朝晖[2] 周晓云[2] HE Huan;ZHENG Zhetang;FU Shuang;YUAN Miaohong;ZHANG Zhaohui;ZHOU Xiaoyun(Lin＇an Environmental Monitoring Station, Hangzhou 311300, China;College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China)

机构地区：[1]临安区环境监测站,浙江杭州311300 [2]浙江工业大学生物与环境工程学院,浙江杭州310014

出　　处：《发酵科技通讯》2018年第1期43-47,共5页Bulletin of Fermentation Science and Technology

基　　金：浙江省科技厅项目(012086)

摘　　要：下喷式液泵型生物反应器作为一种新型高效环流反应器,日益受到有关科研工作者的关注.测试了20L下喷式液泵型生物反应器在不同通风比(0.462,0.615,0.846)和不同循环液速(2.71,2.38,2.01,1.64L/min)下容积氧传递系数K_La的变化规律,并与3L搅拌式生物反应器BioFlo110作对比试验,找到一组能使两个反应器有相近容积氧传递系数(K_La≈173.2h^(-1))的操作条件.试验了_β-葡聚糖酶产生菌黑曲霉315(Aspergillus niger 315)在该反应器内的发酵代谢情况,并与3L搅拌式生物反应器BioFlo110作对比,试验结果表明该反应器产酶效率优于后者,且发酵时间缩短了6h,大幅度节省了能耗.Reverser jet loop reactor（RJL reactor）is a new highly effective circular-current bioreactor,which attracted considerable attention in recent years.The oxygen transfer performance in a 20L RJL reactor was studied.Under different aerial fluxes（0.462,0.615,0.846vvm）and liquid circular rates（2.71,2.38,2.01,1.64 L/min）,their effects on the volume oxygen coefficient KLa were evaluated.The oxygen transfer behavior was compared with that in a 3Luniversal fermenter,BioFlo110.The same KLa（173.2h^-1）in the RJL reactor and BioFlo110could be achieved at a certain combination of aerial flux and liquid circular rate.Under KLaof 173.2h^-1,Aspergillus niger 315（aβ-glucanase producer）was cultivated to produce glucanase in the two reactors.The results showed that the RJL reactor was superior to BioFlo110.The time of producing enzyme was shortened 6hin the RJL reactor,demonstrating a reduced energy consumption.

关 键 词：下喷式液泵型生物反应器 通用发酵罐 容积氧传递系数 发酵 循环液速 

分 类 号：TQ920.5[轻工技术与工程—发酵工程]