超声波协同酶解法提取啤酒花黄酮类的工艺优化及其抗氧化试验  

The memorization of extracting hops flavonoid compounds by enzymolysis approach and antioxidant research

作　　者：王如意[1] 李根[1] 姜柳[1] 乔桢[1] 倪萍[1] 舒刚[1] WANG Ru-yi;LI Gen;JIANG Liu;QIAO Zhen;NI Ping;SHU Gang(College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China)

机构地区：[1]四川农业大学动物医学院,四川成都611130

出　　处：《中国兽医杂志》2018年第2期107-109,共3页Chinese Journal of Veterinary Medicine

基　　金：大学生创新创业训练计划国家级项目"啤酒花浸膏在油脂中抗氧化性能的测定"(1510626054)

摘　　要：为探索超声波协同酶解法提取啤酒值花黄酮的最佳工艺,研究该方法下啤酒花黄酮的抗氧化活性,利用单因素法研究了不同酶量、酶解时间、酶解温度、酶解pH值对超声波协同酶解法提取啤酒花总黄酮含量的影响。结果表明,超声波协同酶解法明显提高啤酒花黄酮的提取率,最佳提取条件为:酶量为2 mg/g,酶解p H值为4,酶解温度30℃,酶解时间2 h,啤酒花总黄酮提取量55.7 mg/g,明显高于传统提取方法的总黄酮提取率38.5%,并对·OH和DPPH具有良好的清除效果,高于常用的抗氧化剂天然维生素C对两者的清除率。Hop known as a perennial herbaceous trailing plant, is rich in flavonoids, and compounds of 2 -phenyl chromone structure. These compounds have antioxidant, anti-cancer, inhibiting lipases, anti-bacterial, anti-inflammatory, anti-mutagenic, blood pressure, detoxification, sedative, diuretic-effects, and many other pharmacological effects. As the development of medicine, the de- mand for hops flavonoids is increasing. The ways of extracting hops from hops flavonoids are mainly the following： ultrasonic extraction method, an organic solvent impregnation, microwave, supercritical fluid extraction, enzymatic hydrolysis, alkaline water extraction and so on. This first attempt was to test the optimum synergy and compare them with organic reagent impregnation and enzymatic method of ultrasonic extraction of hops flavonoids. We found that the latter greatly improved the extraction rate, and the highest rate of flavonoids was 55.7 （mg/g）. Its effect on DPPH and · OH scavenging was 89.3% and 94.3% ,respectively,showing antioxidant effect

关 键 词：啤酒花 黄酮 酶解法 超声波法 抗氧化 

分 类 号：R284[医药卫生—中药学]