圆鮀鲣暗色肉蛋白酶解物的抗疲劳作用  

Anti-fatigue effect of enzymatic hydrolysates from Auxis tapeinosom dark muscle protein

作　　者：陈义明[1] 曹文红[1,2,3,4,5] 章超桦 卢虹玉[1,2,3,4,5] 曾少葵 高加龙[1,2,3,4,5] 郑慧娜 CHEN Yi- ming , CAO Wen-hong , ZHANG Chao-hua, LU Hong-yu, ZENG Shao-kui ,GAO Jia-Iong ,ZHENG Hui-na(1 .College of Food Science and Technology ,Guangdong Ocean University, Zhanjiang 520488, China; 2.Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety,Zhanjiang 520488, China; 3.Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 520488, China ; 4.National Research and Development Branch Center for Shellfish Processing（Zhanjiang） ,Zhanjiang 520488, China; 5.Guangdong higher education institution innovative team of high value processing and utilization of aquatic produets,Zhanjiang 520488, China)

机构地区：[1]广东海洋大学食品科技学院,广东湛江524088 [2]广东省水产品加工与安全重点实验室,广东湛江524088 [3]广东普通高等学校水产品深加工重点实验室,广东湛江524088 [4]国家贝类加工技术研发分中心(湛江),广东湛江524088 [5]广东普通高等学校水产品高值化加工与利用创新团队,广东湛江524088

出　　处：《食品工业科技》2018年第10期315-319,323,共6页Science and Technology of Food Industry

基　　金：广东省科技计划农业攻关团队项目(2011A020102005);广东省科技计划项目(2014B020204001);广东省教育厅创新强校工程项目(2014GKXM047);水产品高值化加工与利用创新团队(GDOU20106030503)

摘　　要：采用行为学和生化指标研究圆鮀鲣暗色肉蛋白酶解物(DMPH)的抗疲劳作用,并且与暗色肉粗酶解物(DMH)进行比较,初步推测其中主要的抗疲劳活性物质。结果表明,DMPH中粗蛋白含量显著高于DMH(p<0.05),DMPH的氨基酸总浓度高于DMH。DMH和DMPH均能显著延长小鼠力竭游泳时间和减少小鼠肝糖原的消耗(p<0.05),能显著降低小鼠血浆乳酸和尿素氮含量(p<0.05),而且还可显著提高小鼠体内抗氧化酶的活力(p<0.05)。DMH和DMPH均具有抗疲劳作用且无显著性差异(p>0.05),而且DMPH中粗蛋白和氨基酸含量高于DMH,初步推断暗色肉中蛋白质水解产生的多肽和氨基酸是酶解物中主要的抗疲劳活性物质。The behavioral and biochemical indexes were used to study the anti-fatigue effect of Auxis tapeinosom dark muscle protein hydrolysates（ DMPH） and were compared with that of dark muscle hydrolysates（ DMH） to preliminarily speculated the main anti-fatigue active substance in DMH. The result showed that the content of crude protein in DMPH was significantly higher than DMH（ P＜0.05） and the total acid concentration was higher than DMH. DMH and DMPH could significantly increase the swimming time of mice and reduce consumption of liver glycogen（ P＜0.05）. They could significantly reduce the content of plasma lactic acid and urea nitrogen in mice and improve the activity of antioxidant enzymes in mice（ P＜0.05）.Both of them had anti-fatigue effect and had no significant differences（ P＜0.05）,and the content of crude protein and total amino acid concentration in DMPH were higher than those of DMH.It could be speculated that amino acids and peptide hydrolysates from dark muscle protein would be the main anti-fatigue active substances in DMH.

关 键 词：圆鮀鲣 暗色肉粗酶解物 暗色肉蛋白酶解物 抗疲劳作用 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]