响应面法优化提取文冠果壳中多酚类物质及其体外抗氧化能力分析  

Response surface methodology optimizing extraction and in vitro antioxidant activity of Xanthoceras sorbifolia polyphenols

作　　者：张雪春 刘江[1] 吴鑫 沈文杰 张婷 张朝秀[1] 姚增玉 戚建华[4] 王振兴[1,3] ZHANG Xuechun1,2, LIU Jiang1, WU Xin3, SHEN Wenjie3, ZHANG Ting3, ZHANG Chaoxiu1, YAO Zengyu4, QI Jianhua4, WANG Zhenxing1,3(1. College of Light Industry and Food Science, Southwest Forestry University, Kunming 650224, China; 2. Key Laboratory of Forest Disaster Warning and Control in Yunnan Province, Southwest Forestry University, Kunming 650224, China; 3. College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China; 4. Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, Chin)

机构地区：[1]西南林业大学轻工与食品工程学院,昆明650224 [2]西南林业大学云南省森林灾害预警与控制重点实验室,昆明650224 [3]江西师范大学生命科学学院,南昌330022 [4]西南林业大学西南山地森林资源保育与利用省部共建教育部重点实验室,昆明650224

出　　处：《中国油脂》2018年第5期117-122,共6页China Oils and Fats

基　　金：云南省教育厅科学研究基金资助性项目(2016ZZX150);云南省农业基础研究联合专项青年项目(2017FG001(-078));江西省产学研合作项目(KJLDI3019);江西师范大学研究生创新基金项目(JYS2016006)

摘　　要：采用超声波辅助提取文冠果壳多酚,以多酚得率为指标,在单因素实验基础上,采用BoxBehnken中心组合实验优化文冠果壳多酚的提取条件;以DPPH自由基清除能力、ABTS^+自由基清除能力、铁还原能力为指标,考察文冠果壳多酚提取物的体外抗氧化能力。结果表明:超声波辅助提取文冠果壳多酚的最佳条件为料液比1∶20、超声时间26.4 min、提取温度47℃、超声波功率250 W,在最佳条件下文冠果壳多酚得率为5.64%;文冠果壳多酚提取物有较强的体外抗氧化能力,其清除DPPH自由基的IC_(50)值为13.36μg/m L,清除ABTS^+自由基的IC_(50)值为23.7μg/m L,铁还原能力为6.4 mg FeSO_4/mg DW,可开发为天然抗氧化剂。In order to optimize the ultrasound - assisted extraction process of Xanthoceras sorbifolia polyphenols, based on single factor experiment, Box -Behnken experimental design and response surface methodology were employed with polyphenols yield as response value. The in vitro antioxidant activity of Xanthoceras sorbifolia polyphenols （XSSC） was evaluated by DPPH free radical scavenging capacity, ABTS ^＋ free radical scavenging capacity and ferric reducing ability. The results showed that the optimal extraction conditions were determined as follows： material - to - liquid ratio 1： 20, ultrasonic time 26.4 min, extraction temperature 47 ℃, ultrasonic power 250 W. Under these conditions, the polyphenols yield was 5.64%. XSSC had excellent in vitro antioxidant activity, the ICsovalues of DPPH free radical scavenging capacity and ABTS ＋ free radical scavenging capacity of XSSC were 13.36, 23.7 μg,/mL respectively, and the ferric reducing ability was 6.4 mg/mg. It had the potential to be explored as a nature antioxidant.

关 键 词：文冠果壳 多酚 体外抗氧化 超声波 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程] ST201.2[轻工技术与工程—食品科学与工程]