高效液相色谱法测定饮料中乙基麦芽酚含量的不确定度评定  

Uncertainty evaluation for determination of ethyl maltol in beverages by high performance liquid chromatography

作　　者：陈毓芳[1,2] 林海丹 官咏仪[1,2] 金梦 张美金[1,2] 钟钰 CHEN Yu-Fang;LIN Hai-Dan;GUAN Yong-Yi;JIN Meng;ZHANG Mei-Jin;ZHONG YH(Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China;Guangdong Provineial Key Laboratory of Import and Export Technieal Measures of Animal, Plant and Food, Guangzhou 510623, China)

机构地区：[1]广东出入境检验检疫局检验检疫技术中心,广州510623 [2]广东省动植物与食品进出口技术措施研究重点实验室,广州510623

出　　处：《食品安全质量检测学报》2018年第9期2175-2180,共6页Journal of Food Safety and Quality

摘　　要：目的评定高效液相色谱法测定饮料中乙基麦芽酚含量的不确定度。方法依据CNAS-GL06《化学分析中不确定度的评估指南》和JJF 1059.1-2012《测量不确定度评定与表示》,分析液相色谱法测定饮料中乙基麦芽酚含量测定过程中的不确定度来源,通过建立数学模型量化不确定度分量,全面分析不确定度来源并进行评估计算。结果本研究测得的饮料中乙基麦芽酚含量为(2.01±0.06)mg/kg,k=2。测定过程的不确定度来源主要有标准曲线配制所使用量具的校准及样品测试过程中重复性实验2项,包括容器和仪器的校准、标准溶液的配制、稀释、定容、工作曲线的拟合和样品的称量、定容和检测重复性等。结论本方法可反映测量的置信度和准确性,为日常实际检验工作提供参考。Objective To evaluate the uncertainty of determination of ethyl maltol in beverage samples by high performance liquid chromatography （HPLC）. Methods According to CNAS-GL06 Guidance on Evaluating the Uncertainty in Chemical Analysis and JJF 1059.1-2012 Evaluation and Expression of Uncertainty in Measurement, the sources of uncertainty in HPLC determination of ethyl maltol in beverages were fully analyzed. Furthermore, the combined uncertainties and expanded uncertainties were calculated based on the established mathematic models, Results The content of ethyl maltol in beverages could be shown as （2.01 ±0.06） mg/kg, k=-2. The uncertainties were mainly attributed to the calibration of measuring tools used in preparation of standard curve, and the repeated experiments in the determination, including containers and instrument calibration, the configuration of the standard solution, dilute, constant volume, working curve fitting and sample weighing, constant volume and repeatability, etc. Conclusion This method accurately reflects the confidence and accuracy of the measurement, which can provide references for the daily actual inspection work.

关 键 词：高效液相色谱法 饮料 乙基麦芽酚 不确定度 

分 类 号：O657.72[理学—分析化学]