响应面法优化褐腐真菌竹生薄孔菌产纤维素酶的液体培养基  

Optimization of Liquid Medium Composition for the Production of Cellulase from Brown Rot Fungus Antrodia bambusicola by Response Surface Methodology

作　　者：马鸿飞 崔宝凯[1] 员瑗 陈圆圆 戴玉成[1] 司静[1] MA Hong-fei;CUI Bao-kai;YUAN Yuan;CHEN Yuan-yuan;DAI Yu-cheng;SI Jing(Institute of Microbiology, Beijing Forestry University, Belting 100083)

机构地区：[1]北京林业大学微生物研究所,北京100083

出　　处：《生物技术通报》2018年第4期91-101,共11页Biotechnology Bulletin

基　　金：中央高校基本科研业务费专项资金资助项目(2017ZY05;2016ZCQ04);中国博士后科学基金(2016T90046)

摘　　要：竹生薄孔菌是一种褐腐真菌,可产生具有重要应用价值的纤维素酶。以纤维素酶为响应值,通过响应面PlackettBurman设计、最陡爬坡设计、Box-Behnken设计建立数学模型,获得竹生薄孔菌产纤维素酶的最适液体培养基为:葡萄糖7.44 g/L、蛋白胨5.0 g/L、麸皮35.31 g/L、Tween 80 9.57 m L/L、KH_2PO_4 1.0 g/L、Mg SO_4·7H_2O 0.5 g/L、Co Cl_2·6H_2O 0.06 g/L、Fe SO_4·7H_2O 0.03g/L、Ca Cl_2 0.5 g/L、Zn SO_4·7H_2O 0.05 g/L、维生素B1 0.01 g/L。在此优化培养基条件下得到的纤维素酶活性为53.17 U/m L,与理论值52.65 U/m L相近,且比优化前提高了2.84倍,说明响应面设计对于优化液体培养基以提高纤维素酶活性切实可行。Antrodia bambusicola,a brown rot fungus,produces cellulase with important practical values. To improve its cellulaseactivity,Plackett-Burman design,the steepest ascent design,and Box-Behnken design in response surface methodology were employed toestablish mathematical model while the cellulase activity was taken as the response. The optimal composition for the production of cellulase fromA. bambusicola was obtained as follows ：glucose 7.44 g/L,peptone 5.0 g/L,wheat bran 35.31 g/L,Tween 80 9.57 m L/L,KH2PO4 1.0 g/L,MgSO4·7 H2O 0.5 g/L,CoCl2·6 H2O 0.06 g/L,FeSO4·7 H2O 0.03 g/L,CaCl2 0.5 g/L,ZnSO4·7 H2O 0.05 g/L,and vitamin B1 0.01 g/L. Under the optimized conditions,the cellulase activity was 53.17 U/m L,which agreed closely with the theoretical result of 52.65 U/m Lpredicted by the developed model,and this yield was 2.84-fold higher than that produced in basal medium alone. Above results indicate that theresponse surface methodology is viable for optimizing medium composition in improving cellulase activity.

关 键 词：褐腐真菌 竹生薄孔菌 纤维素酶 响应面设计 

分 类 号：TQ925[轻工技术与工程—发酵工程]