产ε-聚赖氨酸菌补料分批发酵工艺的研究  

Study on ε-Poly-L-Lysine Producing Strain by Fed-batch Fermentation

作　　者：谢彪[1,2] 唐晓芳 李镇江[1,2] 杨志刚 陈刚[1,2] 郑君 杨晓琴 江源钢 叶莉 XIE Biao;TANG Xiao-fang;LI Zhen-jiang;YANG Zhi-gang;CHEN Gang;ZHENG Jun;YANG Xiao-qing;JIANG Yuan-gang;YE Li(Sichtum Food and Fermentation Industry Research ＆ Design Institute , Chendu Sichuan 611130 ,China;Chengdu finkai Biological Engineering Co. ,Ltd. , Chengdu Sichuan 611130,China)

机构地区：[1]四川省食品发酵工业研究设计院,四川成都610065 [2]成都金开生物工程有限公司,四川成都610065

出　　处：《食品与发酵科技》2018年第3期76-81,共6页Food and Fermentation Science & Technology

摘　　要：探讨了白色链霉菌产ε-聚赖氨酸补料分批发酵工艺中,p H调控方式、碳源、氮源、转速调控方式对ε-聚赖氨酸菌体生长和产物合成的影响。结果表明:通过在发酵中后期控制搅拌转速400r/min,葡萄糖初始浓度为50g/L,氨水流加控制p H为4,流加补料培养基中硫酸铵浓度控制为6%的工艺条件下,可获得ε-聚赖氨酸产量20.6g/L,生物量24.4g/L,分别比优化前提高了155%和37.2%。TThe effects of p H、 carbon source、 nitrogen source and stirrer speed on the batch fermentation and fed-batch fermentation of ε-poly-L-lysine（ε-PL） production were studied. The results showed：in late phase of the fermentation, under the condition of stirrer speed 400 r/min、 controlling p H 4、 the initial glucose was 50 g/L、 the（NH4）2 SO4 feeding was 6%, the production of ε-PL and biomass were finally attained by 20.6 and 24.4 g/L. And the production of ε-PL and the biomass were enhanced 155% and 37.2% compared with that of control.

关 键 词：白色链霉菌 Ε-聚赖氨酸 补料发酵 

分 类 号：TQ922.3[轻工技术与工程—发酵工程]