分心木中化学成分的分离与鉴定  

Separation and Identification of Chemical Constituents from Diaphragma juglandis Fructus

作　　者：王丹[1,2] 董红敬[2] 杨鹏 王晓 耿岩玲 WANG Dan;DONG Hong-jing;YANG Peng;WANG Xiao;GENG Yan-ling(College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China;Key Laboratory of TCM Quality Control Technology ,Shaudong Analysis and Test Center, Qilu University of Technology （Shandong Academy of Sciences） , Jinan 250014, China;Jinan Senkang Sanfeng Biological Engineering Co. ,Ltd,Jinan 250014 ,China)

机构地区：[1]山东农业大学食品科学与工程学院,山东泰安271018 [2]齐鲁工业大学(山东省科学院),山东省分析测试中心,山东省中药质量控制技术重点实验室,山东济南250014 [3]济南森康三峰生物工程有限公司,山东济南250014

出　　处：《食品工业科技》2018年第14期231-234,共4页Science and Technology of Food Industry

基　　金：山东省自然科学基金(ZR2016YL006); 山东省泰山学者岗位专家项目; 山东省科学院先导项目支持

摘　　要：为研究分心木的化学成分,本研究采用大孔树脂(MCI)、反复的反相C18、碱性硅胶、制备液相5种色谱方法对分心木的化学成分进行分离纯化,并通过质谱、核磁共振波谱两种光谱学数据分析鉴定其化学结构。结果表明,从分心木中分离纯化得到9个化合物,分别为:2-乙氧基胡桃醌(1)、胡桃苷A(2)、4,8-二羟基-1-四氢萘醌(3)、4-megastigmen-3,9-dione(4)、3'-O-(E-4-coumaroyl)-quinic acid(5)、二氢红花菜豆酸(6)、5'-O-(E-4-coumaroyl)-quinic acid(7)、香草酸4-O-β-D-葡萄糖苷(8)和litchiol A(9),其中化合物4,5,7,8和9为首次从核桃种属植物中分离得到。本研究对分心木的化学成分有了进一步认识,为探究其药理活性奠定基础,有利于分心木的开发和利用。To explore the chemical constituents of Diaphragma juglandis Fructus.,the compounds were separated and purified by repeated column chromatography on macroporous resin（ MCI）,reversed phase C18,alkaline silica gel and preparative HPLC five methods of chromatography,andtheir chemical structures were identified by spectroscopic data including electrospray ionization mass spectrometry（ ESI-MS） and nuclear magnetic resonance（ NMR）.The results showed that nine compounds were isolated from Diaphragma juglandis Fructus and they were 2-ethoxyjuglone（ 1）,juglanin A（ 2）,isosclerone（ 3）,4-megastigmen-3,9-dione（ 4）,3＇-O-（ E-4-coumaroyl）-quinic acid（ 5）,dihydrophaseic acid（ 6）,5＇-O-（ E-4-coumaroyl）-quinic acid（ 7）,vanillic acid-4-O-β-D-glucopyranoside（ 8） and litchiol A（ 9）.Compounds 4,5,7,8 and 9 were found in Juglans regia L.genus for the first time.The chemical composition of Diaphragma juglandis Fructus had been explored,which would lay the foundation for exploring its pharmacological activity,and be beneficial to the development and utilization.

关 键 词：分心木 化学成分 分离 鉴定 

分 类 号：TS255.6[轻工技术与工程—农产品加工及贮藏工程]