猪血蛋白酶解物对小鼠急性酒精性肝损伤的保护作用  

Porcine Blood Hydrolysate Protect against Acute Alcoholic Liver Injury in Mice

作　　者：胡滨[1] 李康林 吴桥 柯琴 邓玉婵 陈一资[1] HU Bin;LI Kanglin;WU Qiao;KE Qin;DENG Yuchan;CHEN Yizi(College of Food Science,Sichuan Agricultural University,Yaan 625014,China)

机构地区：[1]四川农业大学食品学院,四川雅安625014

出　　处：《食品科学》2018年第11期185-190,共6页Food Science

基　　金：四川省"十二五"重大科技攻关课题(2012NZ001)

摘　　要：目的:研究猪血蛋白酶解物对酒精所致小鼠急性肝损伤的保护作用。方法:小鼠被随机分为空白对照组、模型对照组、药物对照组(还原性谷胱甘肽,每日以20 mg/kg mb灌胃)、猪血蛋白酶解物各剂量组(每日分别以0.83、1.70、3.33 g/kg mb灌胃),连续灌胃30 d,空白对照组和模型对照组给予等体积蒸馏水。第31天,给予体积分数50%乙醇溶液(12 m L/kg mb)建立动物急性肝损伤模型。在灌胃16 h后各组小鼠取血测定谷草转氨酶和谷丙转氨酶活力;处死小鼠后取肝脏测定各项抗氧化指标,并采用组织切片分析小鼠肝损伤程度。结果:与模型对照组相比,猪血蛋白酶解物各剂量组均能降低血清谷丙转氨酶、谷草转氨酶活力及肝脏丙二醛、肿瘤坏死因子-α、白细胞介素-6水平,提高肝脏超氧化物歧化酶、谷胱甘肽过氧化物酶活力及谷胱甘肽水平,使肝脏脂肪变性程度明显减轻。结论:猪血蛋白酶解物对小鼠酒精性肝损伤具有明显保护作用,其机制可能与其抗炎、抗氧化作用有关。Objective： The protective effect of porcine blood hydrolysate（PBH） on acute alcoholic hepatic injury in mice was investigated. Methods： The mice were randomly divided into blank control, model control, reduced glutathione（20 mg/kg mb） and PBH（0.83, 1.70 and 3.33 g/kg mb） groups. All mice except those in the blank control group were administered for 30 days. On day 31, 50% alcohol was given at a dose of 12 m L/kg mb to establish the animal model of acute liver damage. At 16 h post-administration, sera were collected to determine the activities of alanine aminotransferase（ALT） and aspartate transaminase（AST）. All mice were killed by cervical dislocation for the assay of antioxidant enzyme activities in liver, and the degree of hepatic injury was analyzed by histological examination. Results： The levels of AST and ALT in serum and malondialdehyde, tumor necrosis factor alpha and interleukin-6 in liver obviously decreased in the PBH treatment groups when compared with the model control group, while the content of glutathione, and the activities of superoxide dismutase and glutathione peroxidase in liver remarkably increased. The pathological changes such as fatty degeneration in liver were significantly alleviated in the PBH groups. Conclusion： PBH can protect the mouse liver from alcohol-induced injury, and the underlying mechanism may be related to anti-inflammatory and antioxidant activities.

关 键 词：猪血蛋白酶解物 酒精性肝损伤 抗氧化活性 小鼠 

分 类 号：TS209[轻工技术与工程—食品科学]