乙醇对不同菌种合成纤维素的影响及高产菌株对乙醇的敏感性和耐受性  

The Effect of Ethanol on Bacterial Cellulose Synthesis by Different Bacterial Species and the Sensitivity and Tolerance of High-yield Bacterial Strains to Ethanol

作　　者：贾佳 陈华美[1] 王艳梅[1] 邵璐滢 刘晓兰 李从发[1] 刘四新 JIA Jia;CHEN Huamei;WANG Yanmei;SHAO Luying;LIU Xiaolan;LI Congfa;LIU Sixin(College of Food Science and Technology,Hainan Universit;College of Materials and Chemical Engineering,Hainan Universit)

机构地区：[1]海南大学食品学院,海口570228 [2]海南大学材料与化工学院,海口570228

出　　处：《热带生物学报》2018年第2期198-202,共5页Journal of Tropical Biology

基　　金：预发酵椰子水的微生物菌相解析及促进细菌纤维素合成的机理(No.31660485)

摘　　要：为研究细菌纤维素(BC)的高效合成,并探讨影响高效合成稳定性的因素,笔者在Asai合成培养基中添加乙醇,分析其对8个不同菌种合成BC产量的影响,并选出高产菌种,以高产菌株为模型,研究其对乙醇促进性影响的敏感性和耐受性范围。结果表明:不同菌种合成BC对"乙醇影响"的效应不同,其中驹形杆菌属的5个菌种和茂物朝井杆菌(A.bogorensis)都表现出促进效果,以椰冻驹形杆菌(K.nataicola)和木驹形杆菌(K.xylinus)的促进作用最显著(P<0.05),驹形杆菌属的1个未知种(K.sp.)影响不显著(P>0.05),欧洲驹形杆菌(K.europaeus)则表现出抑制作用。筛选到1株"乙醇促进"响应敏感性高且耐受力好的菌株(K.nataicola)Y19,当添加0.5%乙醇时增产6.6倍,添加1%乙醇时产量达最大7.12 g·L-1,增产8.3倍,乙醇最适添加量为1.0%~1.5%。之后,随添加量增加产量增幅降低,直到乙醇添加量达5%时其BC产量才开始低于对照,显示受到抑制,产量降幅为44%。该菌株对"乙醇促进"的浓度响应范围较大,为0.5%~4%。本研究对椰子水自然放置预发酵后用于BC生产的大规模应用具有重要指导意义,筛选到的高效促进和抑制合成的这2种截然相反影响效应的菌株也为后续乙醇影响机制的深入研究奠定了良好基础。In order to study the efficient synthesis of bacterial cellulose（BC） and the factors affecting the stable high yielding BC synthesis, 8 cellulose-producing bacterial species were cultured in Asai chemically-defined medium to observe the effects of ethanol on BC synthesis to select highyielding bacterial species. These highyielding species were used as models to analyze their sensitivity and tolerance to ethanol. The results showed that the effects of ethanol on the 8 cellulose-producing species were quite different in BC synthesis. Of the 8 cellulose-producing species, 5 from the genus Komagataeibacter, and Acetobacter bogorensis were improved in BC synthesis, especially K. nataicola and K. xylinus which had most significant improvement（P 〈 0.05）, while an unknown species from the genus Komagataeibacter was not significantly improved and K. europaeus was inhibited in BC synthesis. A K. nataicola strain Y19 was screened to show high sensitivity and tolerance to ethanol. This strain increased BC yield sharply by 6.6 times in the Asai medium supplemented with 0.5%（similarly hereinafter）ethanol and by 8.3 times to 7.12 g·L-1 at the presence of 1% ethanol, and then declined gradually the growth rate of BC synthesis with the increase of ethanol in the Asai medium. The BC yield of the strain was 44% lower than that of the control when the Asai medium was added with ethanol by up to 5%. This suggested that K. nataicola Y19 had an improving effect in response to ethanol at a wide range of concentrations from 0.5% to 4%, which would explain the vital significance to the large-scale BC production practice by using natural pre-fermented coconut water. On the other hand, one high yield and one inhibition strain were selected, which would lay good foundation for further mechanism study on ethanol effect.

关 键 词：细菌纤维素 乙醇促进作用 驹形杆菌属 茂物朝井杆菌 敏感性 耐受性 

分 类 号：TS201.2[轻工技术与工程—食品科学]