高效液相色谱串联质谱法直接测定功能性饮料中的牛磺酸  

Direct determination of taurine in functional drinks by high performance liquid chromatography tandem mass spectrometry

作　　者：康明芹[1] 陈明岩[1] 王岸英[1] 张蕊蕊 KANG Ming-qin;CHEN Ming-yan;WANG An-ying;ZHANG Rui-rui(Jilin Entry-Exit Inspection and Quarantine Bureau,Changchun 130062,Jilin,China;School of Public Health,Jilin University,Changchun 130021,Jilin,China)

机构地区：[1]吉林出入境检验检疫局,吉林长春130062 [2]吉林大学公共卫生学院,吉林长春130021

出　　处：《粮食与油脂》2018年第7期98-100,共3页Cereals & Oils

基　　金：国家质检总局科技项目(2012IK162;2013IK163)

摘　　要：建立了高效液相色谱串联质谱法(HPLC-MS/MS)直接测定功能性饮料中牛磺酸含量的方法。样品直接稀释后过膜,经BEH Amide(150 mm×2.1 mm,3.5μm)色谱柱分离,以乙腈-水为流动相进行梯度洗脱;负离子模式下测定。结果表明,牛磺酸在0.01~2.0μg/mL范围内与峰面积呈良好线性关系(R=0.9994),加标回收率为96.8%~101.9%,相对标准偏差(RSD)为0.58%~4.2%,方法的定量限为1.0μg/mL。方法与国家标准方法进行了比较,结果表明无显著性差异。The method for the direct contents determination of taurine in functional drinks by high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS） was established. The samples were directly diluted, then fi ltered by membrane, separated by amide column with acetonitrile and water as mobile phase under gradient elution mode, determined by HPLC-MS/MS with negative ion mode. The standard calibration curve showed good linear behavior in the range from 0.01-2.0 μg/mL and the relative coeffi cient was 0.9994. The recovery range was 96.8 %-101.9 % and the RSD range was 0.58 %~4.2 %.The limit of quantitation of the method was 1.0 μg/mL. Comparing with Chinese national standard method, the results showed no signifi cant difference.

关 键 词：牛磺酸 功能性饮料 高效液相色谱串联质谱 

分 类 号：TS207.3[轻工技术与工程—食品科学]