复合酶法提取西兰花老茎不溶性膳食纤维  

Extraction of Insoluble Dietary Fiber from Old Stem of Broccoli by Compound Enzyme Method

作　　者：王崇队 张明[1] 杨立风 范祺 张博华 孟晓峰[1] 马超[1] WANG Chong-dui;ZHANG Ming;YANG Li-feng;FAN Qi;ZHANG Bo-hua;MENG Xiao-feng;MA Chao(Jinan Fruit Research Institute,All China Federation of Supply ＆ Marketing Co-operatives,Jinan 250014,China)

机构地区：[1]中华全国供销合作总社济南果品研究院,山东济南250014

出　　处：《中国果菜》2018年第6期10-14,共5页China Fruit & Vegetable

基　　金：山东省重点研发计划(2017YYSP006);泰山产业领军人才项目(LJNY2015004)

摘　　要：本文以西兰花老茎为原料,采用α-高温淀粉酶和中性蛋白酶复合提取不溶性膳食纤维(IDF)。结果表明:复合酶法提取西兰花老茎不溶性膳食纤维最佳工艺为α-高温淀粉酶添加量7.5μL、酶解温度90℃、pH6、酶解时间60min,中性蛋白酶添加量20μL、酶解温度50℃、pH8、酶解时间60min。在此条件下,西兰花老茎IDF提取率为33.54%。复合酶法提取西兰花老茎不溶性膳食纤维的研究为西兰花老茎资源的综合利用提供了借鉴。The insoluble dietary fiber （IDF） was extracted by using α-high temperature amylase and neutralprotease, using the old stem of broccoli as raw material. The results showed that the addition of α-hightemperature amylase was 7.5μL, the enzymolysis temperature was 90℃, pH6, the time of enzymatic hydrolysiswas 60min; the amount of neutral protease was 20μL, the enzymolysis temperature was 50℃, pH8. Under thiscondition, the extraction rate of IDF was 33.54%. It could provide a theoretical basis for the comprehensiveutilization of broccoli resources.

关 键 词：西兰花 不溶性膳食纤维 α-高温淀粉酶 中性蛋白酶 提取率 

分 类 号：TS255.5[轻工技术与工程—农产品加工及贮藏工程]