EGCG对尼罗罗非鱼肌原纤维蛋白降解的抑制作用  

Inhibition of EGCG on myofibrillar protein degradation of Nile tilapia(Oreochromis niloticus)

作　　者：赵永强[1] 杨贤庆[1] 李来好[1] 吴燕燕[1] 魏涯[1] 岑剑伟[1] 孙祖莉[2] 张红杰[1,3] ZHAO Yongqiang;YANG Xianqing;LI Laihao;WU Yanyan;WEI Ya;CEN Jianwei;SUN Zuli;ZHANG Hongjie(Key Laboratory of Aquatic Product Processing,Ministry of Agriculture National R ＆ D Center for Aquatic Product Processing South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China;College of Life Science,Yantai University,Yantai 264005;College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]中国水产科学研究院南海水产研究所农业部水产品加工重点实验室国家水产品加工技术研发中心,广东广州510300 [2]烟台大学生命科学学院,山东烟台264005 [3]上海海洋大学食品学院,上海201306

出　　处：《水产学报》2018年第8期1307-1314,共8页Journal of Fisheries of China

基　　金：国家现代农业产业技术体系建设专项(CARS-46);国家自然科学基金(31401563;31601533);"十二五"国家科技支撑计划(2015BAK36B06;2015BAD17B03);广州市珠江科技新星专项(201710010167);"扬帆计划"引进创新创业团队专项(2015YT02H109)~~

摘　　要：为研究表没食子儿茶素没食子酸酯(EGCG)对尼罗罗非鱼肌原纤维蛋白在贮藏过程中降解的抑制作用,实验采用质量分数为0.002 5%、0.01%、0.02%、0.03%的EGCG对罗非鱼肌肉进行处理,考察不同浓度EGCG对贮藏过程中罗非鱼肌肉肌原纤维蛋白盐溶性的影响,并采用SDS-PAGE凝胶电泳结合质谱技术筛选EGCG抑制降解靶向蛋白质。结果显示,实验条件下不同浓度的EGCG对肌原纤维蛋白降解均有抑制作用,质量分数为0.01%的EGCG抑制效果最佳,肌原纤维蛋白质含量由贮藏初期的(82.42±0.65)降至第12天的(51.89±0.68)mg/g(下降率37.0%),而对照组由(80.86±1.18)降至(35.33±1.16)mg/g(下降率56.3%)。研究表明,通过SDS-PAGE凝胶电泳及质谱技术鉴定,EGCG对尼罗罗非鱼肌原纤维蛋白降解的抑制作用靶向蛋白质分别为MHC、MLC与肌动蛋白,本结果对罗非鱼冷藏保鲜研究具有一定指导意义。Nile tilapia（Oreochromis niloticus）is an important economic fish species and has been farmed widely in south China.In order to study the inhibitory effect of EGCG on the degradation of myofibrillar protein in tilapia,the tilapia muscle was treated with EGCG with the concentrations of 0.002 5%,0.01%,0.02%and 0.03%respectively.The effects of different concentrations of EGCG on the myofibrillar protein salt solubility of tilapia muscle were investigated during storage.In addition,the targeted proteins of degradation inhibited by EGCG were studied by SDS-PAGE gel electrophoresis and mass spectrometry.The results showed that all of the different concentrations of EGCG had inhibitory effects on myofibrillar degradation,especially when the concentration of EGCG was0.01%,the myofibrillar protein content was decreased from（82.42±0.65）mg/g on day 0 to（51.89±0.68）mg/g on day 12（37.0%of descent rate）,while the myofibrillar protein content of control group was decreased from（80.86±1.18）mg/g to（35.33±1.16）mg/g（56.3%of descent rate）.The results of SDS-PAGE gel electrophoresis and mass spectrometry identification showed that targeted proteins of degradation inhibited by EGCGE were MHC,MLC and actin,respectively.The results of this study have some guiding significance for the preservation research of O.niloticus.

关 键 词：尼罗罗非鱼 EGCG 肌原纤维蛋白 降解 抑制 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]