P-糖蛋白对太平洋牡蛎在镉富集过程中的作用  

Effect of P-glycoprotein on Cadmium Accumulation in Crassostrea gigas

作　　者：李光珍[1] 杨超[1] 刘炳杰[1] 孟祥红[1] 汪东风[1] Li Guangzhen;Yang Chao;Liu Bingjie;Meng Xianghong;Wang Dongfeng(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,Shandon)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《中国食品学报》2018年第7期71-75,共5页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31302162);中国博士后科学基金项目(2013M531652)

摘　　要：以太平洋牡蛎为试验对象,研究不同染Cd浓度和时间条件下太平洋牡蛎体内的Cd含量和P-糖蛋白(P-gp)活性的变化,以及添加P-糖蛋白专一性抑制剂异搏定(Ver)和诱导剂利福平(Rif)对Cd含量的影响。采用火焰原子吸收分光光度法测定Cd含量,排出法测定P-gp的活性变化,结果表明,不同染Cd浓度和染Cd时间对太平洋牡蛎体内的Cd含量及P-gp的活性变化有显著影响,且两者之间的变化相对应,说明P-gp在太平洋牡蛎体内的自身抗Cd机制中发挥作用。添加Ver后,太平洋牡蛎体内Cd含量显著增多,而添加Rif后太平洋牡蛎体内Cd含量下降,说明P-gp在太平洋牡蛎的自身防御机制中发挥重要作用,从而防止机体对外来有害物质的侵入。P-gp抑制Cd在体内的富集,对太平洋牡蛎净化脱Cd,提高其食用安全性具有重要意义。Crassostrea gigas was used as experimental subject, the change of Cd content, P-glycoprotein（P-gp）activity in Crassostrea gigas and adding Verapamil and Rifampicin at different concentrations of Cd and exposure time was studied. The content of Cd and activity of P-gp was determined by the method of flaming atomic absorption and exhaust, respectively. The results showed that Cd concentration and exposure time have great effects on both Cd accumulation and P-gp activity. Verapamil can significantly increase Cd content in Crassostrea gigas, while exposure to Rifampicin has reduced Cd content. It indicated that P-gp played important roles in Cd accumulation, and Rifampicin can effectively reduce Cd content in Crassostrea gigas, which has significant meaning to improve the safety of aquatic products.

关 键 词：太平洋牡蛎 镉 P-糖蛋白 异搏定 利福平 

分 类 号：O657.31[理学—分析化学] TS254.7[理学—化学]