猪源金黄色葡萄球菌lsa(E)基因的流行性研究  

Characterization of the Prevalence and Distribution of Lsa(E) gene in Staphylococcus aureus Isolated from Pig Farms

作　　者：谷立慧 周文渊 王丽[1] 闫鹤[2] GU Li-hui;ZHOU Wen-yuan;WANG Li;YAN He(College of Food Science,South China Agricultural University,Guangzhou 510642,China;Food Science and Engineering School,South China University of Technology,Guangzhou 510640,China)

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]华南理工大学食品科学与工程学院,广东广州510640

出　　处：《现代食品科技》2018年第7期50-55,共6页Modern Food Science and Technology

基　　金："十三五"国家重点研发计划(2016YFD0500606);广东省科技计划项目(2014A020214001;2016A020219001);中央高校基本科研业务费(D2170320);中央高校建设世界一流大学(学科)和特色发展引导专项资金(K5174960)

摘　　要：多重耐药菌的传播对食品安全和人们健康造成严重威胁。为研究介导细菌对林可胺类、截短侧耳素类及链阳菌素A类耐药的lsa(E)基因及其基因簇在猪源金黄色葡萄球菌中的流行情况,对厦门三个猪场分离得到的29株金黄色葡萄球菌进行lsa(E)基因检测,用overlapping PCR对lsa(E)基因阳性菌株进行耐药基因簇的扩增,并用药敏纸片法检测菌株对抗菌药物的敏感性。结果表明,29株金葡菌全部携带lsa(E)基因,且aad E-spc-lsa(E)-lnu(B)-tnp基因簇检出率为96.6%(28/29)。药物敏感实验结果显示,29株lsa(E)基因阳性的金葡菌对克林霉素、克拉霉素、庆大霉素的耐药率分别为100.0%、100.0%、72.4%,对复方新诺明、四环素、环丙沙星的耐药率均为96.6%。未发现有对苯唑西林、喹奴普汀/达福普汀和利奈唑胺耐药的菌株。细菌多重耐药率为100.0%,且耐药谱主要为PEN-GEN-TET-CLA-SXT-CLI-CIP-MXF。研究旨在为临床治疗食源性致病菌引起的食品安全问题和控制多重耐药菌随食品链的传播提供一定的科学依据。The spread of foodborne multi-resistant bacteria poses a serious threat to food safety and public health. The lsa（E） gene confers resistance to lincosamides, pleuromutilinsand streptogramin A. In order to investigate the prevalence and distribution oflsa（E） gene and multi-resistance gene clusters in Staphylococcus aureusisolated from pig farms in Xiamen. The lsa（E） gene and the genetic environment were tested by polymerase chain reaction（PCR） method. Then, lsa（E） positive S. aureus were characterized by drug susceptibility testing. In total, gene cluster： aad E-spc-lsa（E）-lnu（B）-tnp was found in 28 out of 29 lsa（E） positive S. aureus. Resistance was most frequently observed to clindamycin（100.0%）, clarithromycin（100.0%） followed by trimethoprim/sulfamethoxazole（96.6%）, tetracycline（96.6%）, ciprofloxacin（96.6%）, and gentamicin（72.4%）. All the isolates were susceptible to oxacillin, quinupristin-dalfopristin, and linezolid. The multidrug resistance rate was 100.0%, and the main resistance profiles of S. aureus strains was PEN-GEN-TET-CLA-SXT-CLI-CIP-MXF. This study provides a theoretical basis for the prevention and treatment the food quality and safety problem caused by foodborne pathogenic bacteria and the controlling of multi-resistant strains spread of S. aureus in the food chain.

关 键 词：金黄色葡萄球菌 耐药基因 多重耐药 基因簇 

分 类 号：TS201.3[轻工技术与工程—食品科学]