微波辅助提取蛇莓多酚及其体外抗氧化、抑制α-葡萄糖苷酶和乙酰胆碱酯酶能力研究  

Microwave-assisted Extraction,in Vitro Antioxidant Activity,α-glucosidase Inhibition and Acetylcholinesterase Inhibition of Duchesnea indica Polyphenols

作　　者：张雪春[1,2] 刘江 吴鑫 沈文杰 李军 师红玲[2] 王振兴 ZHANG Xue-chun;LIU Jiang;WU Xin;SHEN Wen-jie;LI Jun;SHI Hong-ling;WANG Zhen-xing(Key Laboratory of Forest Disaster Warning and Control in Yunnan Province,Southwest Forestry University,Yunnan Kunming 650224,China;College of Light Industry and Food Science,Southwest Forestry University,Yunnan Kunming 650224,China;Key Laboratory of Functional Small Organic Molecule,Jiangxi Normal University,Jiangxi Nanchang 330022,China)

机构地区：[1]西南林业大学云南省森林灾害预警与控制重点实验室,云南昆明650224 [2]西南林业大学轻工与食品工程学院,云南昆明650224 [3]江西师范大学功能有机小分子教育部重点实验室,江西南昌330022

出　　处：《西南农业学报》2018年第6期1171-1179,共9页Southwest China Journal of Agricultural Sciences

基　　金：云南省农业基础研究联合专项青年项目(2017FG001(-078));云南省教育厅科学研究基金资助性项目(2016ZZX150);江西师范大学研究生创新基金项目(JYS2016006)

摘　　要：【目的】蛇莓为中国民间常用草药,具有良好的生物和药用活性,目前对其抗氧化、降血糖、防治老年痴呆方面研究较少。【方法】以总酚得率为指标,采用响应面法优化蛇莓多酚的微波辅助提取条件;以DPPH自由基清除能力、ABTS+自由基清除能力、铁还原能力为指标,考察其体外抗氧化能力;采用改良的荧光法考察其抑制α-葡萄糖苷酶能力;采用吸收光谱法考察其抑制乙酰胆碱酯酶能力。【结果】微波辅助提取蛇莓多酚的最佳条件为甲醇体积分数67%,料液质量体积比1∶18(W/V),微波功率280 w,微波时间为112 s,在此条件下蛇莓总酚得率为5.21%;蛇莓多酚清除DPPH自由基的IC50值为8.63μg/m L,清除ABTS+自由基的IC50值为32.11μg/m L,铁还原能力为2 190.33μg Fe SO4/mg;抑制α-葡萄糖苷酶的IC50值为223.89μg/m L,低于标准对照物阿卡波糖(310.81μg/m L);抑制乙酰胆碱酯酶的IC50值为202.95μg/m L。【结论】蛇莓多酚有较强的体外抗氧化活性、抑制α-葡萄糖苷酶和乙酰胆碱酯酶的能力,具有开发为天然抗氧化剂和降血糖、防治老年痴呆药物的可能。[ Objective] Duchesnea lndica（DI） is one of the common used herbs in China, it has excellent biological activity and phammcolog- ically active. However, there is less research about anti-oxidant activity, hypoglycemic activity and staving off alzheimer＇ s of DI. [ Method] Response surface methodology（RSM） was employed to optimize extraction conditions by micro-assisted extraction, with the total phenols yield of DI as response values. The in vitro antioxidant activity of DI polyphenol was evaluated by DPPH free radical scavenging capacity, ABTS ＋ free radical scavenging capacity and ferric reducing ability. The α-glucosidase inhibitory activity of DI polyphenol was evaluated by modified fluorescence method, and the acetylcholinesterase （ACHE） inhibitory activity of DI polyphenol was evaluated by absorption spectros- copy. [ Result] The optimized conditions by micro-assisted extraction were determined as follows： methyl alcohol concentration 67 % , sol- vent-to-solid ratio 1：18 （W/V） , microwave power 280 w, microwave time 112 s. Under these conditions, the total phenols yield was 5.21 %. The IC50 value of DPPH free radical scavenging capacity of DI polyphenol was 8.63 Ixg/mL, and the IC50 value of ABTS ＋ free radical scavenging capacity was 32.11 μg/mL, and the ferric reducing ability was 2 190.33 μg FeSO4/mg. The ICs0value of α-glucosidase inhibi- tory activity was 223.89 μg/mL, renmrkably lower than standard control acarbose（ IC50 - 310.81 μg/mL）, the ICs0value of AChE inhibito- ry activity was 202.95 μg/mL. [ Conclusion ] DI polyphenol has excellent in vitro antioxidant activity, α-glucosidase inhibitory activity and AChE inhibitory activity. It has the potential to explore as nature antioxidants, hypoglycemic drags and Alzheimer＇ s drags.

关 键 词：蛇莓 多酚 微波 抗氧化 Α-葡萄糖苷酶 乙酰胆碱酯酶 

分 类 号：S688[农业科学—观赏园艺]