不同栽培基质对糙皮侧耳不同发酵方式下产漆酶活性的影响  

Effects of different culture substrate on laccase activities of Pleurotus ostreatus under different fermentation conditions

作　　者：韩美玲 杜娟 安琪[4] 李丛胜[1] HAN Mei-Ling;DU Juan;AN Qi;LI Cong-Sheng(College of Life Science,Langfang Normal University,Langfang,Hebei 065000,China;Research and Development Center of Edible and Medicinal Fungi of Hebei Provincial Universities,Langfang,Hebei 065000,China;Research Department,Langfang Normal University,Langfang,Hebei 065000,China;lnstitute of Microbiology,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]廊坊师范学院生命科学学院,河北廊坊065000 [2]河北省高校食药用菌资源开发应用技术研发中心,河北廊坊065000 [3]廊坊师范学院科研处,河北廊坊065000 [4]北京林业大学微生物研究所,北京100083

出　　处：《菌物学报》2018年第8期1100-1108,共9页Mycosystema

基　　金：国家自然科学基金青年科学基金(31700024);廊坊市科学技术研究与发展计划项目(2017013180);河北省高校食药用菌应用技术研发中心资助项目(YF201411);廊坊师范学院校级A类创新团队项目(LSCX-A201601)~~

摘　　要：为了比较糙皮侧耳栽培种在不同常规栽培基质上的漆酶活性,分析更适合糙皮侧耳生长的栽培基质,以1株糙皮侧耳Pleurotus ostreatus栽培菌株为研究材料,研究在固态和液态发酵条件下添加木屑、玉米芯和棉籽壳这3种栽培基质后,对其产漆酶活性的影响。结果表明,不同栽培基质对糙皮侧耳漆酶活性具有极显著的影响(P<0.001);不同发酵方法对糙皮侧耳漆酶活性也具有极显著的影响(P<0.001),仅第2天差异不显著。固体发酵与液体发酵条件下,糙皮侧耳在棉籽壳培养基上所检测到的漆酶活性均高于在木屑或者玉米芯培养基上,表明棉籽壳对提高糙皮侧耳漆酶活性的诱导能力更强。此外,糙皮侧耳在棉籽壳培养基上能够快速分泌漆酶,表明棉籽壳对缩短糙皮侧耳漆酶分泌时间的诱导能力更强。A comparative analysis was made between laccase activities of Pleurotus ostreatus cultivated on different conventional substrate, i.e. sawdust, corncob, and cottonseed hull, under solid-state and submerged fermentation conditions for the purposes of selecting most suitable cultivation substrate. The results demonstrated that various culture substrate remarkably affected thelaccase activities of P. ostreatus（P〈0.001）. The laccase activities of P. ostreatus under different fermentation methods were significantly different（P〈0.001）, but no difference was found on the 2 nd day of cultivation（P〈0.05）. Under the conditions of solid-state fermentation and submerged fermentation, the laccase activity detected in synthetic medium containing cottonseed hull was higher than that in synthetic medium containing sawdust or corncob, indicating that the cottonseed hull could be considered as a better inducer for enhancing the laccase activities of P. ostreatus. The synthetic medium containing cottonseed hull was in favour of speeding up the laccase secretion from P. ostreatus, and advancing the time of enzyme production.

关 键 词：糙皮侧耳 栽培基质 发酵方法 漆酶 

分 类 号：TQ925[轻工技术与工程—发酵工程]