超声辅助提取葵花籽黄酮及其抗氧化活性研究  

Ultrasound-assisted extraction of flavonoids from sunflower seed meal and its antioxidant activity

作　　者：张海容[1] 贺泽民 高瑞苑 陈金娥[1] ZHANG Hairong;HE Zemin;GAO Ruiyuan;CHEN Jin＇e(Laboratory of Biochemical Analysis,Xinzhou Teachers University,Xinzhou 034000,Shanxi,China)

机构地区：[1]忻州师范学院生化分析技术研究所,山西忻州034000

出　　处：《中国油脂》2018年第8期133-136,142,共5页China Oils and Fats

基　　金：山西省科技厅软科学研究计划(2017041015-2);山西省教育厅1331工程优势学科化学(2017)资助

摘　　要：研究了超声辅助提取葵花籽黄酮的最佳工艺。通过单因素实验选取超声功率、提取温度、液料比、提取时间为考察因素,利用响应面法Box-Behnken设计对提取葵花籽黄酮工艺参数进行优化,并用DPPH·法评价葵花籽黄酮体外抗氧化活性。结果表明:超声辅助提取葵花籽黄酮的最优工艺参数为超声功率120 W、提取温度64℃、液料比33∶1、提取时间28 min,在此条件下,葵花籽黄酮得率为1.91%;葵花籽黄酮对DPPH·清除率IC50为0.09 mg/m L,优于食品抗氧化剂BHT对DPPH·清除率(IC_(50)为0.11 mg/m L);与传统的索氏提取法对比,超声辅助法提取葵花籽黄酮时间短、节能和得率高。The optimal technology of uhrasound - assisted ied. The ultrasonic power, extraction temperature, liquid extraction of sunflower seed flavonolds was stud- - material ratio and extraction time were select- ed as factors by single factor experiment, and the process parameters of extraction of flavonoids from sun- flower seed meal were optimized by response surface methodology with Box - Behnken design. The results showed that the optimal extraction parameters of flavonoids from sunflower seed meal were obtained as fol- lows： ultrasonic power 120W, extraction temperature 64℃, liquid -material ratio 33：1 and extraction time 28 min. Under these conditions, the sunflower seed flavonoids yield was 1.91%. The IC50 of sun- flower seed flavonoids on DPPH · was 0.09 mg/mL, which was better than that of food antioxidant BHT （ the IC50 was 0.11 mg/mL）. Compared with the traditional Soxhlet extraction method, ultrasound - as- sisted extraction of sunflower seed fiavonoids had the advantages of short time, energy saving and high yield.

关 键 词：葵花籽粕 黄酮 提取 超声辅助 抗氧化活性 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ646[轻工技术与工程—食品科学与工程]