一种中度嗜盐细菌的筛选  

The Isolation of the Moderately Halophilic Bacteria

作　　者：于小青 倪申鹏 姚俊杰 黄志超 吴子辉 陆伽颀 王光强[1] YU Xiao-qing;NI Shen-peng;YAO Jun-jie;HUANG Zhi-chao;WU Zi-hui;LU Jia-qi;WANG Guang-qiang(School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学医疗器械与食品学院,上海200093

出　　处：《食品与发酵科技》2018年第4期55-58,65,共5页Food and Fermentation Science & Technology

基　　金：上海市大学生创新创业项目(SH2016126)

摘　　要：生存于较高浓度盐环境下的中度嗜盐菌在食品及生物技术等方面具潜在的应用价值。为了筛选到该类菌株,本研究将来自上海杨浦区菜市场的泡菜样品稀释过滤后用不同盐浓度的培养基培养,经过反复划线纯化,得到了纯的耐盐菌单菌落。之后,通过菌株16S rRNA PCR、全序列分析等手段,对菌株的16S rRNA的基因序列进行了研究,并确定该菌株属于Staphylococcus piscifermentans。通过使用全自动生长曲线分析仪监测该菌株在不同浓度的氯化钠环境和亚硝酸盐环境下生长曲线。实验发现,Staphylococcus piscifermentans能耐受浓度为180g/L的盐环境和3.0g/L亚硝酸钠环境。Moderately halophilic bacteria that able to live in saline environments offer a lots of potential applications in various fields of food and biotechnology. To obtain the moderately halophilic bacteria, The pickle sample was got from food market in Yangpu, shanghai, and the bacteria was isolated by culturing in medium with various salt concentrations, the single colony was picked out, isolated and purification by streaking. The gene sequencing of the 16 S rRNA of the bacteria continued, that was carried out with the following work, including PCR amplification and clone of the 16 S rRNA and sequence analysis of 16 S rRNA. As a result, the bacteria belongs to Staphylococcus piscifermentans. Then the curve about its growth of the bacteria in different concentrations of sodium chloride environment and nitrite environment was obtained by bioscreen. It was found that Staphylococcus piscifermentans was able to tolerate a salt environment with a concentration of 180 g/L and a sodium nitrite environment with a concentration of 3.0 g/L.

关 键 词：耐盐菌 筛选分离 16S RRNA PCR 氯化钠 亚硝酸钠 

分 类 号：TS201.3[轻工技术与工程—食品科学]