响应面法优化老山芹护绿工艺  

Optimization of Chlorophyll Protection Technology of Heracleum moellendorffii Hance by Response Surface Methodology

作　　者：王炬[1] 张秀玲[1] 高宁[1] 于海鑫 WANG Ju;ZHANG Xiu-ling;GAO Ning;YU Hai-xin(College of Food Science,Northeast Agricultural University,Harbin150030,China)

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《食品工业科技》2018年第17期152-158,166,共8页Science and Technology of Food Industry

基　　金："十三五"国家重点研发计划:东北森林区主要林菜资源生态开发利用技术研发与示范(2016YFC0500307);黑龙江省经济作物(大宗蔬菜)现代农业产业体系岗位专家资金

摘　　要：以老山芹为原料,在单因素实验的基础上,采用响应面法对老山芹护绿剂进行配方优化。选用叶绿素铜钠盐、抗坏血酸、茶多酚浓度为影响因素,以老山芹叶绿素含量和过氧化物酶(POD)活性为响应值进行响应面试验,通过Box-Behnken试验建立二次回归方程模型,分析三种因素及其交互作用对老山芹护绿效果的影响,并对护绿工艺进行优化。结果表明,微波功率600 W处理2 min,老山芹护绿剂最佳配方:0.32 g/L叶绿素铜钠盐、0.95 g/L抗坏血酸、1.68 g/L茶多酚。在此条件下,老山芹叶绿素含量为16.98 mg/g,POD活性为453.54 U·g-1·min-1,相比于未处理组叶绿素含量提高了120%,POD活性下降了69%。三种护绿剂经响应面试验优化后,可以有效地提高老山芹的护色效果,为老山芹未来的加工及工业化生产提供基础性保障。On the basis of single factor experiments, response surface methodology was used to optimize the formulation of chlorophyll protecting agent of Heracleum moellendorffii Hance.The concentrations of sodium copper chlorophyll, ascorbic acid, tea polyphenols as three main influence factors, and chlorophyll content and peroxidase activity as response value, by using response surface test of Design-Expert software, the influence of three factors and their interactions on the effect of chlorophyll protection of Heracleum moeUendorffii Hance was studied, the chlorophyll protection technology was optimized. The results showed that microwave power of 600 W for 2 min, the best optimization formula was：sodium copper chlorophyll 0.32 g/L, ascorbic acid 0.95 g/L, tea polyphenols 1.68 g/L. Under these conditions, the chlorophyll content of Heracleum moellendorffii Hance was 16.98 mg/g, and the aetivity of peroxidase was 453.54 U· g-1· min-1. Compared with the CK group, the content of chlorophyll increased by 120% and POD activity decreased by 69%. The optimized formulation of greening agent could effectively improve the chlorophyll content conservation effect of the Heracleum moellendorffii Hance and provide the basic guarantee for the processing and industrialized production of the Heracleu mmoellendoffii Hance in the future.

关 键 词：老山芹 护绿工艺 叶绿素 过氧化物酶 响应面试验 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]