具有双重抗生素抗性的ε-聚赖氨酸高产菌株选育及生理特性  

Breeding and Physiological Characteristics of ε-Polylysine High-Producing Strain with Double Antibiotic Resistance

作　　者：赵俊杰 张龙[1] 王靓[1] 陈旭升[1] 毛忠贵[1] ZHAO Jun-jie;ZHANG Long;WANG Liang;CHEN Xu-sheng;MAO Zhong-gui(Key Lab of Industrial Biotechnology of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学工业生物技术教育部重点实验室

出　　处：《中国生物工程杂志》2018年第8期59-68,共10页China Biotechnology

基　　金：江苏省产学研合作前瞻性联合研究(BY2016022-25)资助项目

摘　　要：以ε-聚赖氨酸产量为1.60g/L的Streptomyces albulus M-Z18为出发菌株,利用核糖体工程技术选育具有双重抗生素抗性的ε-聚赖氨酸高产菌株,并对高产菌株和出发菌株的生理生化性能进行比较。通过链霉素诱变成功选育出了1株遗传稳定的ε-聚赖氨酸产生菌S.albulus S-7,ε-聚赖氨酸产量为2.03g/L;对S.albulus S-7叠加巴龙霉素,获得1株遗传稳定的具有双重抗性的ε-聚赖氨酸产生菌S.albulus SP-14,ε-聚赖氨酸产量为2.37g/L,比出发菌株S.albulus M-Z18的ε-聚赖氨酸产量增加了48.10%。使用链霉素和巴龙霉素选育具有双重抗生素抗性的ε-聚赖氨酸高产菌株是一种有效的手段。Streptomyces albulus M-Z18 with 1. 60 g/L yield of ε-Poly-L-lysine was used as initial strain to screen high-yield mutant,and the physiological and biochemical properties of high-yield mutant were compared with M-Z18. The ribosomal engineering technology was used to breed the high-yield mutant with double antibiotic resistance. A genetically stable strain named S-7,was successfully screened by streptomycin mutagenesis. The ε-Poly-L-lysine yield of S-7 was 2. 03 g/L. S-7 was used as initial strain to screen mutant with paromomycin,and obtained SP-14,with 2. 37 g/L yield of ε-Poly-L-lysine. The use of streptomycin and paromomycin to breed highyield mutant with high antibiotic resistance is an effective method.

关 键 词：发酵工程 生理生化性能 双重抗生素抗性 Ε-聚赖氨酸 

分 类 号：Q813[生物学—生物工程]