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作 者:衣美艳 郭红 毛毛 YI Meiyan;GUO Hong;MAO Mao(Shandong Oriental Ocean Sci-Tec Co.,Ltd.,Yantai 264003,Shandong,China)
机构地区:[1]山东东方海洋科技股份有限公司,山东烟台264003
出 处:《中国油脂》2018年第9期8-13,共6页China Oils and Fats
基 金:山东省重点研发计划项目(医用食品专项计划)(2017YYSP008)
摘 要:通过酶法提取庸鲽鱼油,对其提取条件及品质进行研究。在单因素试验基础上,以鱼油提取率为指标进行响应面优化试验。结果表明:庸鲽鱼油的最佳提取条件为料液比1∶2(质量比)、加酶量0.25%(以总物料质量计)、动物蛋白酶与胰蛋白酶质量比1∶1.5、p H 7.5、酶解温度52℃、酶解时间2 h,在此条件下庸鲽鱼油提取率可达79.85%;庸鲽粗鱼油的酸值、过氧化值、水分及挥发物、不溶性杂质均达到SC/T 3502—2016粗鱼油一级标准;庸鲽鱼油中饱和脂肪酸含量为67.26%,不饱和脂肪酸含量为33.76%,其中EPA和DHA的总含量为13.62%;庸鲽粗鱼油感官性状达到SC/T 3502—2016精制鱼油标准。庸鲽鱼油是一种具有较高应用价值和开发潜力的新鱼油资源。The oil was extracted from hippoglossus by enzyme method, and the extraction conditions and qualities of hippoglossus oil were analyzed. With the extraction rate of hippoglossus oil as index, the ex- traction conditions were optimized by response surface methodology on the basis of single factor experi- ment. The results showed that the optimal extraction conditions were obtained as follows : mass ratio of sol- id to liquid 1: 2, dosage of enzyme 0.25 % ( based on the mass of total material), mass ratio of animal pro- tease to trypsin 1 : 1.5, pH 7.5, enzymolysis temperature 52 ℃ and enzymolysis time 2 h. Under the opti- mal extraction conditions, the extraction rate of hippoglossus oil reached 79.85 %. The acid value, perox- ide value, insoluble impurities, moisture and volatile matter met the standard of first grade crude oil in SC/T 3502--2016. The content of saturated fatty acids in hippoglossus oil was 67.26%, while the con- tent of unsaturated fatty acids was 33.76%, in which the total content of EPA and DHA was 13.62%. The sensory properties of crude hippoglossus oil met the standard of refined fish oil in SC/T 3502--2016. So hippoglossus oil could be regarded as a kind of new fish oil resource with high application value and development potential.
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