藻蓝蛋白对肺癌LTEP-a-2细胞的体外功能  

The in Vitro Functional of Phycocyanin on Lung Cancer LTEP-a-2 Cells

作　　者：闫燕 郝帅[1] 李爽[1] 赵磊[1] 王成涛 Yan Yan;Hao Shuai;Li Shuang;Zhao Lei;Wang Chentao(Beijing Advanced Innovation Center for Food Nutrition and Human Health,Wang Chengtao;Beijing Technology and Business University,Beijing 100048)

机构地区：[1]北京食品营养与人类健康高精尖创新中心北京工商大学,北京100048 [2]北京市食品添加剂工程技术研究中心,北京100048

出　　处：《中国食品学报》2018年第8期24-32,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31701575,31571801);北京市科技创新服务能力建设-科技成果转化-提升计划项目(PXM2018-014213--000033);北京市优秀人才培养资助青年骨干个人项目(2016000020124G025)：“十三五”时期北京市属高校高水平教师队伍建设支持计划(CIT＆TCD2017fM042)：国家重点研发计划项目(2016YFD0400502);北京市科技计划项目(Z171100002217019)

摘　　要：藻蓝蛋白是一种重要的天然功能性食品添加剂。本文以人类肺癌LTEP-a-2细胞为模型,采用流式细胞术、四甲基偶氮唑蓝(MTT)检测以及划痕愈合等试验,分别从细胞凋亡、细胞增殖和细胞迁移3个方面研究藻蓝蛋白对体外培养的肺癌LTEP-a-2细胞的影响,结果表明,藻蓝蛋白能引起细胞形态发生异常改变;通过上调促凋亡基因和下调抑凋亡基因的表达引起细胞的凋亡;通过异常调控细胞周期G1/S转换点相关基因的表达使细胞阻滞在G1期,同时,藻蓝蛋白通过调控迁移相关基因的表达抑制LTEP-a-2细胞的体外迁移能力。研究结果证实藻蓝蛋白对肺癌LTEP-a-2细胞的体外生长具有显著的抑制作用,这为新型抗肺癌功能食品的开发以及肺癌的治疗提供理论基础。C-phycocyanin （C-PC） is an important natural functional food additive. In order to investigate the effect of C-PC on LTEP-a-2 ceils, we performed flow cytometry, MTT assay and wound healing experiments to explore apop- tosis, proliferation and migration of LTEP-a-2 cells. The results showed that C-PC could cause abnormal changes in cell morphology. It also induced apoptosis by up-regulating expression of pro-apoptotic genes and down-regulating the anti- apoptotic genes. C-PC could regulate the expression of G1/S checkpoint related genes and lead to G1 phase arrest. In addition, it could inhibit the migration of LTEP-a-2 cells in vitro by regulating the expression of migration-related genes. The results indicated that C-PC had a significantly inhibitory effect on the growth of lung cancer LTEP-a-2 cells in vitro, which provided a theoretical basis for the development of new anti-lung cancer functional foods.

关 键 词：藻蓝蛋白 LTEP-α-2细胞 细胞凋亡 细胞增殖 细胞迁移 

分 类 号：TS201.21[轻工技术与工程—食品科学]