百里香酚微乳液抑制白念珠菌的构效关系  

Structure-Activity Relationship of Thymol Microemulsion Inhibiting Candida albicans

作　　者：邓伶俐[1] 康雪帆 阙斐[2] 张辉[1] Deng Lingli;Kang Xuefan;Que Fei;Zhang Hui(College of Biosystems Engineering and Food Science,Full Institute of Food Science,Zhejiang Key Laboratory for Agro-Food Processing,Zhejiang R ＆ D Center for Food Technology and Equipment,Zhejiang University,Hangzhou 310058;Department of Applied Engineering,Zhejiang Economic and Trade Polytechnic,Hangzhou 310018)

机构地区：[1]浙江大学生物系统工程与食品科学学院馥莉食品研究院浙江省农产品加工技术研究重点实验室浙江省食品加工技术与装备工程中心,杭州310058 [2]浙江经贸职业技术学院应用工程系,杭州310018

出　　处：《中国食品学报》2018年第8期86-92,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金面上项目(31471622)

摘　　要：本研究旨在构建可稀释型百里香酚微乳液,研究其微观结构转变及对白念珠菌的抑菌规律。通过小角X射线散射分析发现随着水稀释作用,微乳液逐渐由油包水结构(水含量0~20%)转变为双连续结构(水含量30%~60%),进而变为水包油结构(水含量70%~90%)。粒径分布结果与小角X射线散射结果吻合,其平均粒径小于10 nm。对白念珠菌的动态抑菌试验表明,油包水型百里香酚微乳液在24 h内杀死所有白念珠菌(106 cfu/mL),其双连续型能明显抑制白念珠菌的生长,然而微乳液结构转变为水包油后抑菌能力明显下降。本研究构建了食品级可稀释型百里香酚微乳液,分析其微观结构转变过程,结果发现百里香酚微乳液能够有效抑制白念珠菌的生长,为百里香酚微乳液在食品中的应用提供了新的思路。A food-grade water-dilutable microemulsion system was constructed for encapsulation of thymol. Small Angle X-ray Scattering （SAXS） and dynamic light scattering（DLS） were used to study the microstructure transition of mi- croemulsions with dilution. The dispersion phases gradually inverted from the water-in-oil phase（10%-20%） to the bicon- tinuous phase （30%-60%） and finally to the oil-in-water phase （70%-90%） along the dilution line. The particle size distributions are in good agreement with the SAXS results. The antimicrobial activities of the microemulsions are structural dependent. Microemulsions with W/O structure killed all the Candida albicans within 24 h, and that with bicontinuous structure showed effective in inhibition of Candida dbicans. However, the highly diluted microemulsions with O/W struc- ture didn＇t show inhibition effect of Candida a!bicans. Our work suggests the possibility of applying thymol in the pro- duction of beverages without phase separation. The formation of thymol microemulsion will provide more options for its application in food industry.

关 键 词：百里香酚 微乳液 小角X射线散射 白念珠菌 

分 类 号：TS202.3[轻工技术与工程—食品科学]