一株降胆固醇乳酸菌的筛选及其在模拟消化环境活性的研究  

Screening and study on activity in the simulated gastrointestinal conditions of a cholesterol-lowering Lactic acid bacteria

作　　者：范颖[1] 陈思涵 党芳芳 王喆[1] 满朝新[1] 姜毓君[1,2] FAN Ying;CHEN Sihan;DANG Fangfang;WANG Zhe;MAN Chaoxin;JIANG Yujun(Northeast Agricultural University 1.Key Lab of Dairy Science,Ministry of Education,College of Food Science;National Research Center of Dairy Engineering and Technology,Harbin 150030,China)

机构地区：[1]东北农业大学食品学院乳品科学教育部重点实验室,哈尔滨150030 [2]东北农业大学国家乳业工程技术研究中心,哈尔滨150030

出　　处：《中国乳品工业》2018年第9期4-7,共4页China Dairy Industry

基　　金：黑龙江省杰出青年基金项目(JC201415);黑龙江省院所创新能力提升专项计划项目(YC13D005);东北农业大学学术骨干(15XG26)

摘　　要：通过对来自实验室保藏及分离自西藏地区传统乳制品中的25株乳酸菌体外降胆固醇能力进行比较,筛选出一株降胆固醇能力相对较强的植物乳杆菌TD109,并通过菌落计数,测定了其在模拟消化环境中的耐受性。结果表明:植物乳杆菌TD109的胆固醇脱除率可达47.89%;在pH值为3.0的条件下处理3 h,活菌数可达10~8mL^(-1)以上;在含有1%胆盐的培养基中处理4 h后存活率可达24.06%;在7 g/100 mL的高盐浓度下,菌落总数可达到10~7 cfu/mL以上;并且能够通过胃的消化,耐受肠道环境,在人工胃液、人工肠液分别处理3 h和8 h之后,存活率分别可达13.41%和47.66%,能够顺利定植胃肠道。所以,该菌可用作降胆固醇的潜在益生菌。Lactobacillus plantarum TD109 which has relatively superior cholesterol-lowering effect was screened from 25 Lactic acid bacteria. The activity in the simulated gastrointestinal conditions ofL.plantarum TD109 was also evaluated. The results showed that the cholesterol removal rate ofL.plantarum TD109 was 47.89%. The viable counts can be up to 108 cfu/mL in pH 3.0 for 3 h. The 1% bile salt resistant rate was 24.06%. The viable counts can be up to 107 cfu/mL at sodium chloride of 7 g/100 mL. The tolerance rate in simulated gastric fluid and intestinal fluid were 13.41% and 47.66%, respectively. L.plantarum TD109 can be as the potential probiotics with cholesterol-lowering activity.

关 键 词：乳酸菌 植物乳杆菌 降胆固醇 耐受性 

分 类 号：Q939.117[生物学—微生物学]