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作 者:杨蕾[1] 王柏辉[1] 罗玉龙[1] 王宇 苏琳[1] 赵丽华[1] 靳烨[1] YANG Lei;WANG Bohui;LUO Yulong;WANG Yu;SU Lin;ZHAO Lihua;JIN Ye(College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China)
机构地区:[1]内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特010018
出 处:《食品科学》2018年第19期13-18,共6页Food Science
基 金:国家自然科学基金地区科学基金项目(31660439);"十三五"国家重点研发计划重点专项(2016YFE0106200)
摘 要:以12月龄放牧和圈养条件下的苏尼特羊肌内脂肪、尾脂和肾脂为实验材料,采用实时荧光定量聚合酶链式反应技术,研究饲养方式对苏尼特羊脂肪组织中脂肪代谢相关基因表达量的影响。结果表明:在圈养条件下,硬脂酰辅酶A去饱和酶(stearoyl-coenzyme A desaturase,SCD)基因在脂肪组织中表达量显著高于放牧组(P<0.05);放牧条件下,脂肪酸脱氢酶I(Δ5-fatty acid desaturases,FADS1)、脂肪酸脱氢酶II(Δ6-fatty aciddesaturases,FADS2)、脂肪酸延长酶5(elongaseofverylongchainfattyacid5,Elove5)和过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptor,PPARγ)基因在脂肪组织中表达量高于圈养条件(P>0.05),放牧组脂蛋白脂酶基因在尾脂和肾脂中的表达量高于圈养组(P<0.05)。不同脂肪组织间脂肪代谢相关基因的表达量存在显著差异,在肌内脂肪中FADS1、FADS2、Elove5和脂肪酸β-氧化关键酶肉碱棕榈酰转移酶1(carnitine palmitoyltransferase I,CPT1)基因表达量显著高于尾脂和肾脂(P<0.05);在尾脂和肾脂中PPARγ基因的表达量显著高于肌内脂肪(P<0.05)。脂肪代谢基因相关性研究中,在尾脂中,乙酰辅酶A羧化酶(acetyl-CoA carboxylase,ACC)基因的表达可以极显著促进SCD基因表达(P<0.01),有助于脂肪中单不饱和脂肪酸沉积。The aim of this study was to investigate the effect of different feeding patterns on the expression of lipid metabolism genes in intramuscular, tail and perirenal adipose tissues from 12 month-old Sunit sheep by quantitative real-time polymerase chain reaction (PCR). The results showed that for all three adipose tissues, the expression of stearoyl-CoA desaturase gene (SCD) in stall-fed sheep was significantly higher than in grazing sheep (P 〈 0.05); the expression of Δ5-fatty acid desaturases (FADS1), Δ6-fatty acid desaturases (FADS2), elongase of very long chain fatty acid 5 (Elove5) and peroxisome proliferator-activated receptor (PPARγ) in grazing sheep were higher than in stall-fed sheep (P 〉 0.05). The expression of lipoprotein lipase (LPL) in tail and perirenal adipose tissues from grazing sheep were higher than that stall-fed sheep (P 〈 0.05). The expression of each lipid metabolism gene in different adipose tissues differed significantly. The gene expression of FADS1, FADS2, Elove5 and carnitine palmitoyltransferase I (CPT1) in intramuscular adipose tissue was significantly higher than in the two other adipose tissues (P 〈 0.05), whereas the expression of PPARγ in intramuscular adipose tissue was significantly lower (P 〈 0.05). In tail adipose tissues, the expression of SCD had a significantly positive correlation with the expression of acetyl-CoA carboxylase (ACC) (P 〈 0.01), which was beneficial to the deposition of monounsaturat ed fatty acids in adipose tissues.
关 键 词:饲养方式 苏尼特羊 脂肪代谢基因 实时荧光定量聚合酶链式反应
分 类 号:TS251.5[轻工技术与工程—农产品加工及贮藏工程]
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