杨梅贮藏期真菌的分离鉴定及基于ITS序列的聚类分析  

Separation and identification of fungi from bayberry in storage period and its clustering analysis based on ITS sequence

作　　者：何郁菲[1,2] 刘淑娟 黄浩[1] 赵熙 HE Yu-Fei;LIU Shu-Juan;HUANG Hao;ZHAO Xi(Tea Research Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,China;Tea Testing Center of Hunan Province,Changsha 410125,China)

机构地区：[1]湖南省农业科学院茶叶研究所,长沙410125 [2]湖南省茶叶检测中心,长沙410125

出　　处：《食品安全质量检测学报》2018年第18期4845-4850,共6页Journal of Food Safety and Quality

基　　金：湖南省农业科学院青年创新引导项目(2016QN31)~~

摘　　要：目的分离幵鉴定杨梅贮藏期真菌。方法通过稀释涂布平板分离法以及划线纯化,从贮藏期杨梅果实中分离、纯化真菌菌种,幵结合传统形态学与ITS (internal transcribed spacer)全序列分析对其进行准确鉴定。结果最终鉴定出共14株真菌,其中1株毛霉(Mucorracemosus)、1株白地霉(Geotrichumcandidum)、1株腐皮壳属霉菌(Diaporthesp.)、1株好食链孢霉(Neurosporasitophila)、3株出芽短梗霉(Aureobasidium pullulans)、 4株酵母菌(Pichiakudriavzevii,Pichiaterricola,Hanseniasporaopuntiae,Saccharomycopsis crataegensis)以及3株青霉(Penicillium sp., Penicillium expansum, Penicillium herquei)。从进化关系上看有3类显示较近的亲缘关系,第1类为青霉属,第2类为出芽短梗霉,第3类为毕赤酵母。结论结合传统形态学与ITS全序列分析可对杨梅贮藏期真菌进行更为准确的鉴定。Objective To separate and identify the fungi from bayberry in storage period. Methods The fungal strains were isolated and purified from bayberry during storage period by the spread plate method and streak plate method. And it was accurately identified by the traditional morphology and ITS （internal transcribed spacer） sequence analysis. Results A total of 14 strains of fungi were identified, including 1 strain ofMucor racemosus, 1 strain of Geotrichum candidum, 1 strain of Diaporthe sp., 1 strain of Neurospora sitophila, 3 strains of Aureobasidium pullulans, 4 strains of yeast （Pichia kudriavzevii, Pichia terricola, Hanseniaspora opuntiae, Saccharomycopsis crataegensis） and 3 strains of Penicillium （Penicillium sp., Penicillium expansum, Penicillium herquei）. From the evolutionary relationship 3 categories showed close relationship. The first category was Penicillium, the second category was Aureobasidium pullulans, and the third was Pichia. Conclusion Combining traditional morphology and ITS full sequence analysis can more accurately identify fungi in storage of bayberry.

关 键 词：杨梅 贮藏期 真菌 ITS序列分析 聚类分析 分离 鉴定 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]