高效液相色谱法同时检测黄油及无水奶油中苯甲酸、山梨酸、安赛蜜、糖精钠  

Simultaneous determination of benzoic acid, sorbic acid, acesulfame and saccharin sodium in butter and anhydrous butter by high pressure liquid chromatography

作　　者：袁凤琴[1] 王佳[1] 李照[1] 张利俊 孙佳美 徐向峰[1] 常建军[1] 宋晓东[1] YUAN Feng-Qin;WANG Jia;LI Zhao;ZHANG Li-Jun;SUN Jia-Mei;XU Xiang-Feng;CHANG Jian-Jun;SONG Xiao-Dong(Inner Mongolia Mengniu Dairy Industrial Co.,Ltd.,Hohhot 011500,China)

机构地区：[1]内蒙古蒙牛乳业(集团)股份有限公司中心实验室,呼和浩特011500

出　　处：《食品安全质量检测学报》2018年第19期5133-5137,共5页Journal of Food Safety and Quality

摘　　要：目的建立黄油及无水奶油中2种甜味剂(安赛蜜、糖精钠)及2种防腐剂(苯甲酸、山梨酸)含量的高效液相色谱分析方法。方法样品去除脂肪后,用甲醇-水(1:1,V:V)提取,提取液使用C8色谱柱(250mm×4.6 mm, 5μm)、在波长为230 nm、流动相为1.0 mL/min的条件下进行检测。结果苯甲酸、山梨酸、安赛蜜、糖精钠在0～100μg/mL范围内,浓度与峰面积有良好的线性关系,相关系数大于0.99,检出限分别为0.87、0.91、2.75、2.91 mg/kg;样品的加标平均回收率分别为93.12%～102.34%、93.02%～103.23%、91.53%～108.16%、93.48%～105.46%,相对标准偏差均小于10%。结论本方法可适用于黄油及无水奶油中苯甲酸、山梨酸、安赛蜜、糖精钠含量同时测定。Objective To establish a method for the determination of 2 sweeteners（acesulfame and saccharin sodium） and 2 preservatives（benzoic acid and sorbic acid） in butter and anhydrous cream by high performance liquid chromatography（HPLC）. Methods After the fat was removed from the sample, the mixture of methanol and water（1：1=V：V） was used as an extracting agent. The extracting solution was analyzed in C8 chromatographic column by HPLC under the conditions of 230 nm in wavelength and 1.0 mL/min in the speed of mobile phrase. Results Benzoic acid, sorbic acid, acesulfame and saccharin sodium had good linear relationships in the range of 0-100 μg/kg and the correlation coefficients were larger than 0.99. The limits of detection were 0.87, 0.91, 2.75, 2.91 mg/kg, respectively. The average standard recoveries of samples were 93.12%-102.34%, 93.02%-103.23%, 91.53%-108.16%, 93.48%-105.46%, respectively. The relative standard deviations were less than 10%. Conclusion This method can be used for simultaneous determination of benzoic acid, sorbic acid, acesulfame and saccharin sodium in butter and anhydrous butter.

关 键 词：苯甲酸 山梨酸 安赛蜜 糖精钠 高效液相色谱法 同时检测 

分 类 号：O657.72[理学—分析化学] TS227[理学—化学]