甲状腺乳头状癌中PD-L1蛋白表达与BRAF V600E基因突变的相关性  

Correlation between PD-L1 protein expression and BRAF V600E gene mutation in papillary thyroid carcinoma

作　　者：罗华荣[1,2] 甘梅富 徐铖 徐伟铭 梁勇 LUO Huarong;GAN Meifu;XU Cheng;XU Weiming;LIANG Yong(The First Clinical Medical College,Wenzhou Medical University,Whenzhou,325035;Department of Pathology,Enze Hospital,Taizhou Enze Medical Center（Group）,Taizhou,318050;Medical College of Taizhou University,Taizhou,318000)

机构地区：[1]温州医科大学第一临床医学院,浙江温州325035 [2]台州恩泽医疗中心(集团)恩泽医院病理科,浙江台州318050 [3]台州学院医学院,浙江台州318000

出　　处：《温州医科大学学报》2018年第10期718-722,共5页Journal of Wenzhou Medical University

基　　金：浙江省自然科学基金资助项目(LY14H160036);台州市科技计划项目(1701KY56)

摘　　要：目的:探讨甲状腺乳头状癌(PTC)中PD-L1蛋白表达与BRAF V600E基因突变的相关性。方法:应用免疫组织化学Envision两步法检测74例PTC及癌旁正常甲状腺组织的PD-L1蛋白表达,Q-PCR法检测PTC组织中BRAF V600E基因突变情况,分析PD-L1蛋白表达与PTC各临床病理特征及BRAF V600E基因突变的相关性。结果:PD-L1阳性表达于48.6%(36/74)的PTC组织中,高于癌旁对照组(P<0.01)。PD-L1蛋白在PTC中的表达与年龄、肿瘤大小、淋巴细胞性甲状腺炎(CLT)背景、肿瘤浸润淋巴细胞(TILs)以及临床分期有关(P<0.05)。BRAF V600E基因突变的阳性率为79.7%(59/74)。BRAF V600E基因突变与PTC的组织学分型有关(P<0.01)。PD-L1蛋白表达与BRAF V600E基因突变之间无明显相关性(Kappa=-0.091,P>0.05)。多因素logistic回归分析显示CLT背景是影响PD-L1蛋白表达的独立因素(OR=18.675,95%CI:3.074～113.453,P<0.01)。结论:PD-L1蛋白在PTC组织中有过量表达,其阳性表达与PTC的临床病理特征有关,而与BRAF V600E基因突变无关。Objective： To investigate the correlation between PD-L1 protein expression and BRAF V600E gene mutation in papillary thyroid carcinoma （PTC）. Methods： 74 cases of PTC and normal thyroid tissue speci-mens were used to detect PD-L1 protein expression by immunohistochemical EnVision two step method. Gene mutation of BRAF V600E was detected by Q-PCR. And the correlation between PD-L1 protein expression and the clinicopathological parameters of PTC including BRAF V600E gene mutation was evaluated. Results： The positive rate of PD-L1 protein expression was 48.6% （36/74） in PTC tissues, signifcantly higher than that in con-trol group （P〈0.01）. The expression of PD-L1 protein was correlated with age, tumor size, chronic lymphocytic thyroiditis （CLT） background, tumor-infltrating lymphocytes （TILs） and clinical stage （P〈0.05）. The positive rate of BRAF V600E gene mutation was 79.7% （59/74） in PTC tissues. The gene mutation of BRAF V600E was correlated with histological classifcation of PTC （P〈0.01）. Consistency analysis showed that there was no rela-tionship between PD-L1 protein expression and BRAF V600E gene mutation （Kappa=-0.091, P〉0.05）. Among these parameters, background CLT was independent factor affecting PD-L1 protein expression （OR=18.675, 95%CI： 3.074-113.453, P〈0.01）. Conclusion： PD-L1 protein is over-expressed in PTC tissues. The expression of PD-L1 protein is correlated with clinicopathologic features of PTC, but there is no relationship between PD-L1 protein expression and BRAF V600E gene mutation in PTC.

关 键 词：甲状腺乳头状癌 PD-L1 BRAF V600E 基因突变 

分 类 号：R736.1[医药卫生—肿瘤]