产类胡萝卜素菌株的筛选鉴定及功能基因分析  

Screening and Identification of a Carotenoids-producing Strain and Its Functional Genes Analysis

作　　者：彭龙云 谢能中[2] 蒙健宗[1] 刘伟丰[3] PENG Longyun;XIE Nengzhong;MENG Jianzhong;LIU Weifeng(Guangxi University,College of life science and technology,Nanning 530004,China;Guangxi Academy of Sciences,Nanning 530007,China;Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China)

机构地区：[1]广西大学生命科学与技术学院,广西南宁530004 [2]广西科学院,广西南宁530007 [3]中国科学院微生物研究所,北京100101

出　　处：《酿酒》2018年第5期69-72,共4页Liquor Making

摘　　要：湖南酃酒是一类传统地方工艺酿造的米酒,酿造过程中独特的微生物类群对酃酒的风味及功能物质含量具有重要影响,对其中的微生物进行鉴定、分离,并筛选具有特定功能的菌株,有助于研究酃酒的酿造工艺、口味及功能,并获得有潜在应用价值的微生物。研究从湖南地方酃酒酒糟中分离一株产色素的微生物,对菌株进行常规形态和生化特征分析;利用16SrDNA序列分析对该菌株进行序列对比鉴定,将其鉴定为泛菌属Pantoea sp,命名为FL03。确认该菌能够产多种类胡萝卜素物质,其中包含有虾青素合成基因crtZ及crtW。将FL03的虾青素合成基因crtZ及crtW克隆到重组载体上,通过在已经含有虾青素前体基因簇的底盘大肠杆菌中进行表达,成功异源合成虾青素产物,产量可达3.72mg/g(DCW)。这一工作为研究酃酒的发酵工艺、分析相关微生物类群的功能及应用价值打下基础。In this study, we isolated a pigment-producing microbial strain from Lingwine, a kind of traditional rice wine in Hunan province. The strain was analyzed using morphologic and biochemical methods. Then16 srDNA sequence was determined and compared with online datebase. The strain was identified as Pantoea sp. We named the strain as FL03. Pigment analysis study indicated FL03 could produce various carotenoids. The astaxanthin synthesis genes, crtZ and crtW, were then identified. We constructed crtZ and crtW genes into E. coli expression vectors and transformed it into precursor chassis. In this strain, astaxanthin yield can be up to 3.72 mg/g（DCW）.

关 键 词：枸杞 葡萄 发酵 勾兑 

分 类 号：TS262.4[轻工技术与工程—发酵工程] TS261.15[轻工技术与工程—食品科学与工程]