绿豆活性肽对HepG2肝癌细胞增殖的抑制作用  

Inhibition of Mung Bean Peptides on Proliferation in HepG2 Cell

作　　者：李梅青[1,2] 王康 周鑫[1] Li Meiqing;Wang Kang;Zhou Xin(School of Tea and Food Science,Anhui Agricultural University,Hefei 230036;Hefei Processing Institute for Agriculture,Hefei 230036)

机构地区：[1]安徽农业大学茶与食品科技学院,合肥230036 [2]合肥农产品加工研究院,合肥230036

出　　处：《中国食品学报》2018年第10期52-57,共6页Journal of Chinese Institute Of Food Science and Technology

摘　　要：分析绿豆分离蛋白的基本成分,采用木瓜蛋白酶酶解制备绿豆活性肽,通过CCK-8法和显微镜观察,研究绿豆活性肽对HepG2人肝癌细胞增殖活性及细胞形态的影响,并利用高效液相色谱法对其分子质量分布进行测定。结果表明,绿豆分离蛋白的蛋白质含量为91.4%;不同浓度的绿豆活性肽对HepG2细胞生长均有明显的抑制作用,且存在时间效应和剂量效应,48 h和72 h的IC50分别为4.106, 2.148 mg/mL;随着绿豆活性肽作用时间的延长,HepG2细胞逐渐变圆、漂浮,特征形态消失;绿豆活性肽成分较为复杂,分子质量较小且呈不规则分布,主要集中在1 000 u以下,占总量的80.42%,而分子质量在3 000 u以上的部分只占3.27%。绿豆活性肽的体外肿瘤抑制活性显著,具有开发利用的价值。Based on the basic components were analyzed, the mung bean protein isolate was treated by papain to obtain mung bean peptides. CCK-8 method and microscope were used to study the inhibitory effect of mung bean peptides on proliferation activity and cell morphology of HepG2 cell, and the molecular weight of mung bean peptides were detected by HPLC. The results show that the main components of mung bean protein isolate are protein which content as high as 91.4%; cell toxicity experiment show that the inhibition of different concentrations of mung bean peptides on the proliferation of HepG2 cell was remarkable and existed in dose-and time-dependent manners. The IC50 of 48 h and 72 h is 4.106 mg/mL and 2.148 mg/mL respectively; microscopy revealed show that with the increasing of action time, HepG2 cells became round, floating, morphological characteristics disappear. Detection of molecular weight showed that the components of mung bean peptides are complex, molecular weight is small and distributed irregularly, mainly below 1 000 u,accounted for 80.42% of the total, and the part which molecular weight more than 3 000 u only accounted for 3.27%.These results show that mung bean peptides have strong anti-cancer activity in vitro and has the further development prospect.

关 键 词：绿豆活性肽 HEPG2细胞 抑制作用 分子质量分布 

分 类 号：TS201.4[轻工技术与工程—食品科学]