人era的结构特点分析及其定点突变体的构建  

Structural analysis of human era and construction of its site-directed mutants

作　　者：纪宗玲[1] 陈苏民[1] 陈南春[1] 吴元明[1] 刘继中[2] 路凡[1] 张俊杰[1] 

机构地区：[1]第四军医大学生物化学与子生物学教研室,陕西西安710032 [2]第四军医大学西京医院全军骨科研究所,陕西西安710032

出　　处：《免疫学杂志》2002年第5期325-328,共4页Immunological Journal

基　　金：国家自然科学基金 (39870 38030 0 0 0 0 91 );全军医药卫生科研基金 (98M1 0 8)资助项目

摘　　要：目的　构建最近克隆的人era基因的定点突变体。方法　利用数据库对era的结构特点进行分析 ,在此基础上用改良的重叠延伸法分别构建人EraN端和C端的定点突变体。结果　获得了分别针对人EraN端TGP结合结构域 (位于2 9 - 36氨基酸残基 )和C端具有RNA结合活性的KH结构域 (位于 2 97- 340氨基酸残基 )的定点突变体。Objective To construct site directed mutants of human era.Methods The structural character of human era was analyzed by computer,era mutants were constructed by improved overlap extention method.Results Three site directed mutants were constructed successfully,one sited within GTP binding domain and the other two within KH domain.Conclusion These results may shed light on further functional study of human Era.

关 键 词：结构特点 定点突变体 构建 ERA基因 基因突变 

分 类 号：R392[医药卫生—免疫学]