茶树根系Actin基因克隆及表达分析  

Gene Cloning and Expression Analysis of Actin in Tea Plant Root

作　　者：李远华[1] 陆建良[2] 范方媛[2] 石玉涛 

机构地区：[1]武夷学院茶与食品学院/福建省武夷茶资源创新利用重点实验室/福建省高校茶叶工程研究中心/中国乌龙茶产业协同创新中心(培育),福建武夷山354300 [2]浙江大学茶叶研究所,浙江杭州310058

出　　处：《茶叶科学》2015年第4期336-346,共11页Journal of Tea Science

基　　金：国家自然科学基金面上项目(31070613);福建省科技厅重点项目(2012N0025);国家级大学生创新创业训练计划项目(201310397001)

摘　　要：采用SSH技术分析了VA菌根处理后福鼎大白茶根系基因差异表达情况,获得了差异序列,序列比对显示,在下调表达序列中可能包含了10种未知功能的基因;在上调表达序列中可能包含了5种可能的基因。采用RACE技术获得了Actin基因全长序列,Actin基因长1 606 bp(Gen Bank,登录号KJ946252),具有1 131bp开放阅读框(1st^1 131st),编码377个氨基酸。分子生物信息学分析表明,Actin蛋白分子量约30.69 k D,等电点为5.27,定位于细胞核等亚细胞区位。研究还显示,Actin在不同品种中表达无显著差异,对非生物性胁迫响应也较弱。By using SSH, we analyzed differences in gene expression of root from Fuding white tea infected by VA mycorrhiza and obtained diversity sequences. The sequence alighment showed that the down-regulated expression sequence possibly contained 10 unknown genes and the up-regulated expression sequence possible contained 5 known genes. The Actin genic full-length sequence was obtained by using RACE. The length of Actin gene was 1 606 bp(Gen Bank Accession No. KJ946252), with 1 131 bp ORF(1st-1 131st), the sequence encoded 377 amino acid. Bioinformatics indicated that the Actin protein's molecular weight was about 30.69 k D, IEP was 5.27, located in subcellular fraction area like cell nucleus. The study also showed Actin expressed no obvious difference in different cultivars and it responded weak to non-biological stress.

关 键 词：茶树 ACTIN 基因克隆 表达 

分 类 号：S571.1[农业科学—茶叶生产加工] Q943.2[农业科学—作物学]