平滑肌瘤女性患者中肿瘤坏死子-α-308启动子和p53密码子72基因的多态性  

Tumor necrosis factor-α-308 promoter and p53 codon 72 gene polymorphisms in women with leiomyomas

作　　者：HsiehY.-Y. Tsai F.-J. 刘淑娟 

出　　处：《世界核心医学期刊文摘（妇产科学分册）》2005年第4期33-34,共2页Core Journal in Obstetrics/Gynecology

摘　　要：Tumor necrosis factor-α(TNF-α), a proinflammatory cytokine, plays an important role in the process of autoimmune diseases. p53 is related to the regulation of cell growth and prevention of carcinogenesis. We propose to investigate whether gene polymorphisms for TNF-α-308 promoter and p53 could be used as markers of susceptibility in leiomyomas. Prospective basic study. Departments of gynecology and genetics in a medical center. Group 1: leiomyoma (n = 159); group 2: nonleiomyoma (n = 131). Genomic DNA was obtained from peripheral leukocyte. The TNF-αand p53 gene polymorphisms were amplified by polymerase chain reaction (PCR), enzyme restriction, and electrophoresis. Two gene polymorphisms were identified: [1] the A (cuttable)/G (uncuttable) polymorphisms of the TNF-αgene on chromosome 6p21.3; [2] A (cuttable)/P (uncuttable) polymorphisms of the p53 gene on chromosome 17p. Genotype and allelic frequencies in both groups were compared. Genotype distribution and allele frequency of TNF-α.gene polymorphism in both groups were significantly different. Proportions of A homozygote/heterozygote/G homozygote for TNF-α.in both groups were: (group 1) 61%/34.6%/4.4%and (group 2) 81.7%/14.5%/3.8%. Proportions of allele A/G for TNF-αin both groups were: (group 1) 78.3%/21.7%and (group 2) 88.9%/11.1%. Distributions of p53 polymorphisms in both groups were not different. The proportions of A homozygotes/heterozygotes/P homozygotes for p53 were (group 1) 32.7%/42.1%/25.2%and (group 2) 28.2%/48.9%/22.9%. G homozygote and G allele for TNF-αpromoter are related to a higher risk of leiomyomas. The p53 codon 72 gene polymorphism is not associated with the susceptibility of leiomyomas.Tumor necrosis factor-α(TNF-α), a proinflammatory cytokine, plays an important role in the process of autoimmune diseases. p53 is related to the regulation of cell growth and prevention of carcinogenesis. We propose to investigate whether gene polymorphisms for TNF-α-308 promoter and p53 could be used as markers of susceptibility in leiomyomas. Prospective basic study. Departments of gynecology and genetics in a medical center. Group 1: leiomyoma (n = 159); group 2: nonleiomyoma (n = 131). Genomic DNA was obtained from peripheral leukocyte. The TNF-αand p53 gene polymorphisms were amplified by polymerase chain reaction (PCR), enzyme restriction, and electrophoresis. Two gene polymorphisms were identified: [1] the A (cuttable)/G (uncuttable) polymorphisms of the TNF-αgene on chromosome 6p21.3; [2] A (cuttable)/P (uncuttable) polymorphisms of the p53 gene on chromosome 17p. Genotype and allelic frequencies in both groups were compared. Genotype distribution and allele frequency of TNF-α.gene polymorphism in both groups were significantly different. Proportions of A homozygote/heterozygote/G homozygote for TNF-α.in both groups were: (group 1) 61%/34.6%/4.4%and (group 2) 81.7%/14.5%/3.8%. Proportions of allele A/G for TNF-αin both groups were: (group 1) 78.3%/21.7%and (group 2) 88.9%/11.1%. Distributions of p53 polymorphisms in both groups were not different. The proportions of A homozygotes/heterozygotes/P homozygotes for p53 were (group 1) 32.7%/42.1%/25.2%and (group 2) 28.2%/48.9%/22.9%. G homozygote and G allele for TNF-αpromoter are related to a higher risk of leiomyomas. The p53 codon 72 gene polymorphism is not associated with the susceptibility of leiomyomas.

关 键 词：平滑肌瘤 P53 密码子 启动子 多态性 聚合酶链反应 限制性酶切 纯合子 自身免疫性疾病 遗传学科 

分 类 号：R730.2[医药卫生—肿瘤]