黄海黄杆菌YS-9412-130低温碱性蛋白酶活性必需基团分析  

Analysis of functional essential groups of marine low-temperature alkaline protease from Flavobacterium yellowsea YS-9412-130

作　　者：洪义国[1] 孙谧[1] 修朝阳[2] 张云波[1] 王海英[1] 王跃军[1] 

机构地区：[1]中国水产科学研究院黄海水产研究所,青岛266071 [2]中国科学院上海生物工程研究中心,上海200233

出　　处：《海洋水产研究》2002年第3期26-29,共4页Marine Fisheries Research

基　　金：国家"8 63"项目 (863 -819-0 6-0 1)资助

摘　　要：用化学修饰法结合酶的紫外吸收光谱变化研究黄海黄杆菌 YS- 94 12 - 130分泌的海洋低温蛋白酶的功能基团性质 ,结果表明 ,羟基、咪唑基、ε-氨基及胍基均与酶活性有关 ,而羧基、巯基与酶活性无关。酶的性质与丝氨酸蛋白酶有很大的相似性。The character of functional essential groups of marine low temperature alkaline protease from Flavobacterium yellowsea YS 9412 130 was studied with chemical modify and ultraviolet absorb method. The result shows that Ser, His, Lys, Arg are closely related to the activity of the enzyme. But carboxyl and sulfhydryl have no relation with the activity of the enzyme. The character of the enzyme is similar to Serine protease.

关 键 词：黄海黄杆菌 低温碱性蛋白酶 必需基团 酶活力 化学修饰法 

分 类 号：Q556.9[生物学—生物化学]